Linked Life Data

8173590

Source:http://linkedlifedata.com/resource/pubmed/id/8173590

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-6-6
pubmed:databankReference
pubmed:abstractText
Casein kinase 2 (CK-2) is a ubiquitous messenger-independent protein serine/threonine kinase that has been implicated in the control of cell growth and proliferation. By employing androgen action in the prostate as a model of growth control, we have previously documented that androgens modulate prostatic CK-2 activity in a tissue specific manner. Here we have investigated the role of transcriptional control of prostatic CK-2 in androgenic regulation of its activity. We first cloned and sequenced full length cDNAs encoding the alpha and beta subunits of rat CK-2. The cDNA sequence encoding the alpha subunit corresponded with previously reported sequences for other species, as well as with the derived partial amino acid sequence reported for a rat cDNA. The cloned cDNA for rat CK-2-beta subunit, not reported previously, was also identical in amino acid sequence to that of other species. The cDNAs for alpha and beta subunits were employed as probes to examine the effects of altered androgenic status on transcription of mRNAs for the subunits of prostatic CK-2. Androgen deprivation caused a slow decline in transcription of the a subunit, so that no change was noted at 24 h postcastration; however, at later periods of androgen deprivation a progressive but relatively slow decline was apparent. Administration of a single dose of 5 alpha-dihydrotestosterone (5 alpha-DHT) to 6-d castrated animals did not elicit an early expression of new mRNAs for CK-2-alpha and beta subunits. However, a significant expression of mRNAs for the two subunits was apparent at 8 h (i.e., later stages of the prereplicative phase) reaching a peak in the proliferative phase of prostatic growth (i.e., at 24-48 h) following androgen administration. These data suggest that androgenic regulation of CK-2 gene transcription is not an early event related to androgen action, but is substantial in the prereplicative phase of prostatic cell proliferation mediated by androgen. Further, androgenic stimulation of the mRNA expression for the alpha and beta subunits of CK-2 appears to be differential.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0968-8773
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-62
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8173590-Amino Acid Sequence, pubmed-meshheading:8173590-Androgens, pubmed-meshheading:8173590-Animals, pubmed-meshheading:8173590-Base Sequence, pubmed-meshheading:8173590-Casein Kinases, pubmed-meshheading:8173590-Cloning, Molecular, pubmed-meshheading:8173590-DNA, Complementary, pubmed-meshheading:8173590-Dihydrotestosterone, pubmed-meshheading:8173590-Gene Expression Regulation, pubmed-meshheading:8173590-Male, pubmed-meshheading:8173590-Molecular Sequence Data, pubmed-meshheading:8173590-Orchiectomy, pubmed-meshheading:8173590-Prostate, pubmed-meshheading:8173590-Protein Biosynthesis, pubmed-meshheading:8173590-Protein Conformation, pubmed-meshheading:8173590-Protein Kinases, pubmed-meshheading:8173590-RNA, Messenger, pubmed-meshheading:8173590-Rats, pubmed-meshheading:8173590-Rats, Sprague-Dawley, pubmed-meshheading:8173590-Sequence Analysis, DNA
pubmed:year
1993
pubmed:articleTitle
Cloning of cDNAs encoding the alpha and beta subunits of rat casein kinase 2 (CK-2): investigation of molecular regulation of CK-2 by androgens in rat ventral prostate.
pubmed:affiliation
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.