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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1994-6-2
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pubmed:abstractText |
The Duchenne muscular dystrophy gene gives rise to transcripts of several lengths. These mRNAs differ in their coding content and tissue distribution. The 14 kb mRNA encodes dystrophin, a 427 kDa protein found in muscle and brain, and the short transcripts described encode DP71, a 77 kDa protein found in various organs. These short transcripts have many features common to the deduced primary structure of dystrophin, especially in the cysteine-rich specific C-terminal domains. The dystrophin C-terminal domain could be involved in membrane anchorage via the glycoprotein complex, but such a functional role for these short transcript products has yet to be demonstrated. Here we report the first isolation of a short transcript product from saponin-solubilized cardiac muscle membranes using alkaline buffer and affinity chromatography procedures. This molecule was found to be glycosylated and could be easily dissociated from cardiac muscle and other non-muscle tissues such as brain and liver. DP71-specific monoclonal antibody helped to identify this molecule as being related to the dystrophin gene family. Immunofluorescence analysis of bovine or chicken cardiac muscle showed a periodic distribution of DP71 in transverse T tubules and this protein was co-localized with the dystrophin glycoprotein complex in the Z-disk area.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1300197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1301145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1319059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1377655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1380160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1406935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1461283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1471976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1478922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1577159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1913804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-1986002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2026615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2081733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2176467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2180744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2185033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2236001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2268434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2407739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2481825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2541343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2648158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-2746654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-3282674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-3287171,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-3607877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-3652206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-7411166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-8094772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-8344426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-8418988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8172595-8482362
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
299 ( Pt 2)
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pubmed:geneSymbol |
DMD
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
359-65
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8172595-Amino Acid Sequence,
pubmed-meshheading:8172595-Animals,
pubmed-meshheading:8172595-Antibodies, Monoclonal,
pubmed-meshheading:8172595-Base Sequence,
pubmed-meshheading:8172595-Blotting, Western,
pubmed-meshheading:8172595-Brain,
pubmed-meshheading:8172595-Cattle,
pubmed-meshheading:8172595-Cell Membrane,
pubmed-meshheading:8172595-Chickens,
pubmed-meshheading:8172595-DNA Primers,
pubmed-meshheading:8172595-Dystrophin,
pubmed-meshheading:8172595-Humans,
pubmed-meshheading:8172595-Liver,
pubmed-meshheading:8172595-Membrane Glycoproteins,
pubmed-meshheading:8172595-Molecular Sequence Data,
pubmed-meshheading:8172595-Molecular Weight,
pubmed-meshheading:8172595-Muscular Dystrophies,
pubmed-meshheading:8172595-Myocardium,
pubmed-meshheading:8172595-Organ Specificity,
pubmed-meshheading:8172595-Polymerase Chain Reaction,
pubmed-meshheading:8172595-RNA, Messenger,
pubmed-meshheading:8172595-Rats,
pubmed-meshheading:8172595-Restriction Mapping,
pubmed-meshheading:8172595-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Characterization and localization of a 77 kDa protein related to the dystrophin gene family.
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pubmed:affiliation |
INSERM U.300, Faculté de Pharmacie, Montpellier, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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