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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1994-6-1
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pubmed:abstractText |
Cytosolic Ca2+ overload may play a key role in the process of lead-induced retinal injury and degeneration. We report that retinal calcium content was elevated following developmental and in vitro lead exposure. To determine the concentration-dependent effects of Ca2+ (5-1000 nM) on retinal mitochondrial bioenergetics an isolation procedure was developed. Isolated mitochondria were efficiently coupled; had good respiratory control ratios with the NAD-linked substrates, glutamate or pyruvate plus malate (G/M or P/M), and the FAD-linked substrate, succinate plus rotenone (S/R); and possessed a Na+/Ca2+ exchanger. The major finding was that at equimolar [Ca2+] > or = 35 nM, mitochondria were more sensitive to and exhibited a greater degree of inhibition of coupled and uncoupled respiration with NAD-linked substrates compared to S/R. At all [Ca2+], decreases in State 3 and uncoupled respiration were similar, thereby eliminating the ATP synthase and ADP/ATP translocase as sites of inhibition and suggesting that opening the mitochondrial permeability transition pore (MTP) did not contribute to the inhibition. The effects of toxicological [Ca2+] were: (1) blocked by ruthenium red, (2) blocked by dibucaine only in the presence of NAD-linked substrates, and (3) partially reversed by NAD+ with G/M after opening the MTP. Results with G/M suggest that Ca2+ acts on the inner membrane phospholipase A2 to decrease NADH CoQ reductase activity and/or produce a NAD+ leak, whereas with S/R, Ca2+ may inhibit succinate dehydrogenase. In conclusion, Ca2+ inhibits retinal mitochondrial ATP production, which may contribute to the retinal cell injury and death observed in developmentally lead-exposed rats.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0041-008X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
309-21
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8171438-Adenosine Triphosphate,
pubmed-meshheading:8171438-Animals,
pubmed-meshheading:8171438-Calcium,
pubmed-meshheading:8171438-Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone,
pubmed-meshheading:8171438-Energy Metabolism,
pubmed-meshheading:8171438-Female,
pubmed-meshheading:8171438-Lead,
pubmed-meshheading:8171438-Microscopy, Electron,
pubmed-meshheading:8171438-Mitochondria,
pubmed-meshheading:8171438-Oxidative Phosphorylation,
pubmed-meshheading:8171438-Oxygen Consumption,
pubmed-meshheading:8171438-Rats,
pubmed-meshheading:8171438-Rats, Inbred Strains,
pubmed-meshheading:8171438-Retina,
pubmed-meshheading:8171438-Subcellular Fractions,
pubmed-meshheading:8171438-Substrate Specificity
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pubmed:year |
1994
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pubmed:articleTitle |
Substrate-dependent effects of calcium on rat retinal mitochondrial respiration: physiological and toxicological studies.
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pubmed:affiliation |
College of Optometry, University of Houston, Texas 77204-6052.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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