Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-6-1
pubmed:abstractText
Following encounter with antigen, the immunoglobulin genes in B lymphocytes undergo somatic hypermutation. Most nucleotide substitutions are introduced into a region flanked by the V gene promoter and intron enhancer. Experiments described here using transgenic mice revealed that the V kappa promoter does not contain specific signals since hypermutation was retained on substituting it by a beta-globin promoter. However, both the kappa intron and kappa 3' enhancer regions were found to be essential for full hypermutation. This dependence of hypermutation on both enhancers contrasts with transgene expression in hybridomas in which only the 3' enhancer (and not the intron enhancer) is necessary to achieve high mRNA levels. The results show that full hypermutation depends on multiple elements, removal of some of which may drastically impair but not totally abolish the process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
239-48
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8168132-Amino Acid Sequence, pubmed-meshheading:8168132-Animals, pubmed-meshheading:8168132-B-Lymphocytes, pubmed-meshheading:8168132-Base Sequence, pubmed-meshheading:8168132-Cloning, Molecular, pubmed-meshheading:8168132-DNA, pubmed-meshheading:8168132-DNA Mutational Analysis, pubmed-meshheading:8168132-Enhancer Elements, Genetic, pubmed-meshheading:8168132-Gene Expression, pubmed-meshheading:8168132-Gene Rearrangement, B-Lymphocyte, Light Chain, pubmed-meshheading:8168132-Genes, Immunoglobulin, pubmed-meshheading:8168132-Hybridomas, pubmed-meshheading:8168132-Immunoglobulin Variable Region, pubmed-meshheading:8168132-Immunoglobulin kappa-Chains, pubmed-meshheading:8168132-Introns, pubmed-meshheading:8168132-Mice, pubmed-meshheading:8168132-Mice, Transgenic, pubmed-meshheading:8168132-Molecular Sequence Data, pubmed-meshheading:8168132-Nuclear Matrix, pubmed-meshheading:8168132-Peyer's Patches, pubmed-meshheading:8168132-Point Mutation, pubmed-meshheading:8168132-Polymerase Chain Reaction
pubmed:year
1994
pubmed:articleTitle
Elements regulating somatic hypermutation of an immunoglobulin kappa gene: critical role for the intron enhancer/matrix attachment region.
pubmed:affiliation
Medical Research Council Laboratory of Molecular Biology, Cambridge, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't