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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1994-5-27
|
| pubmed:abstractText |
Metabolic activity of a gel-entrapment, hollow fiber, bioartificial liver was evaluated in vitro and during extracorporeal hemoperfusion in an anhepatic rabbit model. The bioartificial liver contained either 100 million rat hepatocytes (n = 12), fibroblasts (n = 3), or no cells (n = 7) during hemoperfusion of anhepatic rabbits. Eight other anhepatic rabbits were studied without hemoperfusion as anhepatic controls, and three sham rabbits served as normal controls. Albumin production rates (mean +/- SEM) were similar during in vitro (17.0 +/- 2.8 micrograms/h) and extracorporeal (18.0 +/- 4.0 micrograms/h) application of the hepatocyte bioartificial liver. Exogenous glucose requirements were reduced (p < 0.01) and euglycemia was prolonged (p < 0.001) in anhepatic rabbits treated with the hepatocyte bioartificial liver. The maximum rate of glucose production by the hepatocyte bioartificial liver ranged from 50-80 micrograms/h. Plasma concentrations of aromatic amino acids, proline, alanine, and ammonia were normalized in anhepatic rabbits during hepatocyte hemoperfusion. Gel-entrapped hepatocytes in the bioartifical liver performed sulfation and glucuronidation of 4-methylumbelliferone. P450 activity was demonstrated during both in vitro and extracorporeal application of the BAL device by the formation of 3-hydroxy-lidocaine, the major metabolite of lidocaine biotransformation by gel-entrapped rat hepatocytes. In summary, a gel-entrapment, bioartificial liver performed multiple hepatocyte-specific functions without adverse side effects during extracorporeal application in an anhepatic, small animal model. With its potential for short term support of acute liver failure, scale-up of the current bioartificial liver device is indicated for further investigations in large animal, preclinical trials.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Albumins,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Gels,
http://linkedlifedata.com/resource/pubmed/chemical/Hymecromone,
http://linkedlifedata.com/resource/pubmed/chemical/Lidocaine,
http://linkedlifedata.com/resource/pubmed/chemical/Urea
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0963-6897
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
441-52
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8167929-Albumins,
pubmed-meshheading:8167929-Amino Acids,
pubmed-meshheading:8167929-Animals,
pubmed-meshheading:8167929-Artificial Organs,
pubmed-meshheading:8167929-Biotransformation,
pubmed-meshheading:8167929-Blood Cell Count,
pubmed-meshheading:8167929-Blood Glucose,
pubmed-meshheading:8167929-Cells, Cultured,
pubmed-meshheading:8167929-Collagen,
pubmed-meshheading:8167929-Equipment Design,
pubmed-meshheading:8167929-Extracorporeal Circulation,
pubmed-meshheading:8167929-Gels,
pubmed-meshheading:8167929-Hemoperfusion,
pubmed-meshheading:8167929-Hepatectomy,
pubmed-meshheading:8167929-Hymecromone,
pubmed-meshheading:8167929-Lidocaine,
pubmed-meshheading:8167929-Liver,
pubmed-meshheading:8167929-Male,
pubmed-meshheading:8167929-Rabbits,
pubmed-meshheading:8167929-Rats,
pubmed-meshheading:8167929-Rats, Sprague-Dawley,
pubmed-meshheading:8167929-Urea
|
| pubmed:articleTitle |
Extracorporeal application of a gel-entrapment, bioartificial liver: demonstration of drug metabolism and other biochemical functions.
|
| pubmed:affiliation |
Department of Surgery, University of Minnesota, Minneapolis 55455.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|