Linked Life Data

8167560

Source:http://linkedlifedata.com/resource/pubmed/id/8167560

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1994-6-2
pubmed:abstractText
A new Ph1-positive acute lymphoblastic leukemia cell line, designated as ALL/MIK, has been developed from a patient with Ph1-positive acute leukemia. The ALL/MIK cells showed an immunophenotype of common ALL with rearranged JH and Jk genes. The ALL/MIK cells showed no M-bcr rearrangement using Southern blot analysis with either 3' or 5' M-bcr probes, but had the bcr gene rearrangement on bcr-2 within the first intron of the bcr gene. Consistent with this result, the reverse transcriptase-dependent polymerase chain reaction (RT-PCR) assay revealed that the ALL/MIK cells contained the transcript derived fusion of the first exon of bcr gene and the second exon of abl gene. Although the ALL/MIK cells were defined as early pre-B cells by immunophenotypical and genotypical analyses, they were capable of differentiating into monocytoid lineage by when cultured with TPA. Furthermore, another Ph1-positive ALL cell line, (TOM-1), was investigated for its ability to differentiate to monocytoid lineage. TOM-1 was also induced to monocytoid lineage by TPA. Thus, the present study suggested that the leukemic transformation in some Ph1-positive ALL may occur at the level of multipotential hematopoietic cells capable of differentiating towards lymphoid and myelo-monocytoid lineage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1042-8194
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:geneSymbol
bcr-2, bcr-abl
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
287-96
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8167560-Aged, pubmed-meshheading:8167560-Base Sequence, pubmed-meshheading:8167560-Blotting, Northern, pubmed-meshheading:8167560-Blotting, Southern, pubmed-meshheading:8167560-Cell Differentiation, pubmed-meshheading:8167560-Cell Line, pubmed-meshheading:8167560-Culture Techniques, pubmed-meshheading:8167560-DNA, Neoplasm, pubmed-meshheading:8167560-DNA Primers, pubmed-meshheading:8167560-Exons, pubmed-meshheading:8167560-Female, pubmed-meshheading:8167560-Fusion Proteins, bcr-abl, pubmed-meshheading:8167560-Gene Expression, pubmed-meshheading:8167560-Gene Rearrangement, pubmed-meshheading:8167560-Genes, Immunoglobulin, pubmed-meshheading:8167560-Humans, pubmed-meshheading:8167560-Karyotyping, pubmed-meshheading:8167560-Molecular Sequence Data, pubmed-meshheading:8167560-Monocytes, pubmed-meshheading:8167560-Oncogene Proteins, pubmed-meshheading:8167560-Oncogenes, pubmed-meshheading:8167560-Philadelphia Chromosome, pubmed-meshheading:8167560-Polymerase Chain Reaction, pubmed-meshheading:8167560-Precursor Cell Lymphoblastic Leukemia-Lymphoma, pubmed-meshheading:8167560-Protein-Tyrosine Kinases, pubmed-meshheading:8167560-Proto-Oncogene Proteins, pubmed-meshheading:8167560-Proto-Oncogene Proteins c-bcr, pubmed-meshheading:8167560-RNA, Neoplasm, pubmed-meshheading:8167560-Restriction Mapping, pubmed-meshheading:8167560-Transcription, Genetic, pubmed-meshheading:8167560-Tumor Cells, Cultured
pubmed:year
1994
pubmed:articleTitle
Establishment and characterization of a new Ph1-positive ALL cell line (ALL/MIK) presenting bcr gene rearrangement on bcr-2 and ALL-type bcr/abl transcript: suggestion of in vitro differentiation to monocytoid lineage.
pubmed:affiliation
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't