Linked Life Data

8162967

Source:http://linkedlifedata.com/resource/pubmed/id/8162967

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-5-25
pubmed:abstractText
The effects of sequential combinations of N-methylformamide (NMF) with adriamycin (ADM) on a human melanoma cell line (M14) were investigated. The results obtained can be summarized as follows: (i) When NMF was administered before ADM (NMF-->ADM), a decrease of ADM-induced cytotoxicity and intracellular ADM content was revealed. Conversely, the reverse combination (ADM-->NMF) produced a remarkable reduction of cell survival accompanied by a significant intracellular drug retention. (ii) The fluorescence microscopy revealed that in NMF- and in NMF-->ADM-treated cells the actin microfilament distribution appeared to be very similar to that in control cells. On the contrary, a disorganization of the microfilament architecture was observed in ADM-->NMF-treated cells. (iii) The microtubule organization was significantly altered by NMF. This effect appeared to be even more evident in M14 cells treated with the combination ADM-->NMF. (iv) Confocal microscopy observations showed an intracytoplasmic retention of ADM in ADM-->NMF-treated cells. These findings suggest that the cytoskeletal changes induced by NMF might interfere with the ADM efflux mechanisms, accounting for the high drug level and low survival detected in ADM-->NMF-treated cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-4800
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Effects of sequential combinations of N-methylformamide with adriamycin on cultured melanoma cells (M14).
pubmed:affiliation
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't