Linked Life Data

8160960

Source:http://linkedlifedata.com/resource/pubmed/id/8160960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1994-5-19
pubmed:abstractText
The effects of bis- and mono-intercalating dyes on the capillary electrophoretic separation of double-stranded DNA have been investigated in buffers containing (hydroxypropylmethyl)-cellulose. Broad bands and incomplete separations of phi X 174 DNA HaeIII digest were obtained with the bis-intercalators ethidium homodimer 1 (EthD-1) and ethidium homodimer 2 (EthD-2), under a wide range of dye and polymer concentrations. The above results were attributed to the presence of several intramolecular dye/DNA complexes for each fragment. For EthD-2/DNA complexes, but not for EthD-1/DNA complexes, additional bands beyond the expected number of fragments were observed. The extra bands were attributed to formation of intermolecular dimers. Use of the monomeric intercalating dyes, ethidium and propidium, allowed complete separation of all fragments of phi X 174 DNA HaeIII digest. A low-power green He-Ne laser (543.6 nm) was shown to be satisfactory for laser-induced fluorescence detection with any of these dyes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1168-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Separation of nucleic acids by capillary electrophoresis in cellulose solutions with mono- and bis-intercalating dyes.
pubmed:affiliation
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.