Linked Life Data

8159980

Source:http://linkedlifedata.com/resource/pubmed/id/8159980

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-5-19
pubmed:abstractText
This paper reviews recent studies done in the author's laboratory on molecular mechanisms of nickel genotoxicity, using as an experimental model the teratogenic effects of bivalent nickel ions (Ni2+) in South Africa frogs (Xenopus laevis). A Ni(2+)-binding protein, pNiXa, was identified in Xenopus oocytes and embryos (molecular weight 45 kDa, isoelectric point approximately 8.5) with a strong homology to human alpha 1-antitrypsin, alpha 1-antichymotrypsin, and other serine proteinase inhibitors. CNBr peptides of pNiXa showed sequence identity to Ep45. Nondenatured pNiXa, purified by nickel affinity chromatography, inhibits bovine alpha 1-chymotrypsin. The possibility that pNiXa plays a key role in Ni2+ teratogenesis is indicated by (i) the avidity of pNiXa for Ni2+, (ii) the presence of pNiXa when the embryos are susceptible to Ni2+ teragenesis, and (iii) the potential of the (HX)n-motif to form Ni2+ complexes that could catalyze the formation of oxygen free radicals and thereby damage deoxyribonucleic acid (DNA) and chromosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0355-3140
pubmed:author
pubmed:issnType
Print
pubmed:volume
19 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-80
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Search for molecular mechanisms in the genotoxicity of nickel.
pubmed:affiliation
Department of Laboratory Medicine, University of Connecticut Medical School, Farmington 06030.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review, Research Support, Non-U.S. Gov't