Linked Life Data

8159879

Source:http://linkedlifedata.com/resource/pubmed/id/8159879

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-5-17
pubmed:abstractText
Hormonal quantitation has had important developments since Yalow and Berson created the first radioimmunoassay for insulin in 1959. Since then much research has been done to find new non-isotopic methods which offer better levels of sensitivity, specificity and precision than RIA. DELFIA (dissociation enhancement lanthanide fluoroimmunoassay) is a new alternative technology which is a specific time resolved fluoroimmunoassay (TR-FIA) which combines higher purified antigens, monoclonal antibodies in a sandwich type assay with long fluorescence decay time of lanthanide chelates as europium, samarium and terbium used as labels, and a particular time resolving fluorometer for nanoseconds measurements. As part of the validation and introduction of the DELFIA method in our laboratory we made a comparative evaluation of DELFIA and RIA in the quantification of serum luteinizing hormone and follicle stimulating hormone. The data favor the DELFIA method in terms of sensitivity, specificity, volume of sample, half life of reagents, as well as the elimination of radioactive tracers.
pubmed:language
spa
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0034-8376
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
579-84
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:articleTitle
[Comparison of the DELFIA and RIA methods for measuring luteinizing and follicle stimulating hormones in serum].
pubmed:affiliation
Departamento de Biología de la Reproducción, Instituto Nacional de la Nutrición Salvador Zubirán, México, D.F.
pubmed:publicationType
Journal Article, Comparative Study, English Abstract