Linked Life Data

8158122

Source:http://linkedlifedata.com/resource/pubmed/id/8158122

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-5-18
pubmed:abstractText
The nuclear factor kappa B (NF-kappa B) is a eukaryotic transcription factor. In B cells and macrophages it is constitutively present in cell nuclei, whereas in many other cell types, NF-kappa B translocates from cytosol to nucleus as a result of transduction by tumor necrosis factor alpha (TNF alpha), phorbol ester, and other polyclonal signals. Using neuroblastoma cell lines as models, we have shown that in neural cells NF-kappa B was present in the cytosol and translocated into nuclei as a result of TNF alpha treatment. The TNF alpha-activated NF-kappa B was transcriptionally functional. NF-kappa B activation by TNF alpha was not correlated with cell differentiation or proliferation. However, reagents such as nerve growth factor (NGF) and the phorbol ester phorbol 12-myristate 13-acetate (PMA), which induce phenotypical differentiation of the SH-SY5Y neuroblastoma cell line, activated NF-kappa B, but only in that particular cell line. In a NGF-responsive rat pheochromocytoma cell line, PC12, PMA activated NF-kappa B, whereas NGF did not. In other neuroblastoma cell lines, such as SK-N-Be(2), the lack of PMA induction of differentiation was correlated with the lack of NF-kappa B activation. We found, moreover, that in SK-N-Be(2) cells protein kinase C (PKC) enzymatic activity was much lower compared with that in a control cell line and that the low PKC enzymatic activity was due to low PKC protein expression. NF-kappa B was not activated by retinoic acid, which induced morphological differentiation of all the neuroblastoma cell lines used in the present study. Thus, NF-kappa B activation was not required for neuroblastoma cell differentiation. Furthermore, the results obtained with TNF alpha proved that NF-kappa B activation was not sufficient for induction of neuroblastoma differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1716-26
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8158122-Animals, pubmed-meshheading:8158122-Base Sequence, pubmed-meshheading:8158122-Cell Differentiation, pubmed-meshheading:8158122-Cell Line, pubmed-meshheading:8158122-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:8158122-Humans, pubmed-meshheading:8158122-Molecular Sequence Data, pubmed-meshheading:8158122-NF-kappa B, pubmed-meshheading:8158122-Nerve Growth Factors, pubmed-meshheading:8158122-Neuroblastoma, pubmed-meshheading:8158122-Nuclear Proteins, pubmed-meshheading:8158122-Oligodeoxyribonucleotides, pubmed-meshheading:8158122-PC12 Cells, pubmed-meshheading:8158122-Protein Kinase C, pubmed-meshheading:8158122-Recombinant Proteins, pubmed-meshheading:8158122-T-Lymphocytes, pubmed-meshheading:8158122-Tetradecanoylphorbol Acetate, pubmed-meshheading:8158122-Transcription, Genetic, pubmed-meshheading:8158122-Transfection, pubmed-meshheading:8158122-Tretinoin, pubmed-meshheading:8158122-Tumor Cells, Cultured, pubmed-meshheading:8158122-Tumor Necrosis Factor-alpha
pubmed:year
1994
pubmed:articleTitle
Activation of nuclear factor kappa B in human neuroblastoma cell lines.
pubmed:affiliation
Laboratoire d'Immunologie Cellulaire et Tissulaire, CNRS URA 625, Hôpital de la Pitié Salpêtrière, Paris, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't