Linked Life Data

8157683

Source:http://linkedlifedata.com/resource/pubmed/id/8157683

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1994-5-19
pubmed:databankReference
pubmed:abstractText
The human lysosomal cysteine proteinases, cathepsins H, L, and B, have been mapped to chromosomes 15, 9, and 8, respectively, and the genomic structures of cathepsins L and B have been determined. We report here the chromosomal localization and partial gene structure for a recently sequenced human cysteine proteinase, cathepsin S. A 20-kilobase pair genomic clone of the human cathepsin S gene was isolated from a human fibroblast genomic library and used to map the human cathepsin S gene to chromosome 1q21 by fluorescence in situ hybridization. This clone contains exons 1 through 5, introns 1 through 4, part of intron 5, and > 7 kilobase pairs of the 5'-flanking sequence. The gene structure of human cathepsin S is similar to that of cathepsin L through the first 5 exons, except that cathepsin S introns are substantially larger. Sequencing of the 5'-flanking region revealed, similar to human cathepsin B, no classical TATA or CAAT box. In contrast to cathepsin B, cathepsin S contains only two SP1 and at least 18 AP1 binding sites that potentially could be involved in regulation of the gene. This 5'-flanking region also contains CA microsatellites. The presence of AP1 sites and CA microsatellites suggest that cathepsin S can be specifically regulated. Results of Northern blotting using probes for human cathepsins B, L, and S are consistent with this hypothesis; only cathepsin S shows a restricted tissue distribution, with highest levels in spleen, heart, and lung. In addition, immunostaining of lung tissue demonstrated detectable cathepsin S only in lung macrophages. The high level of expression in the spleen and in phagocytes suggests that cathepsin S may have a specific function in immunity, perhaps related to antigen processing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11530-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8157683-Animals, pubmed-meshheading:8157683-Base Sequence, pubmed-meshheading:8157683-Cathepsin L, pubmed-meshheading:8157683-Cathepsins, pubmed-meshheading:8157683-Chromosome Mapping, pubmed-meshheading:8157683-Chromosomes, Human, Pair 1, pubmed-meshheading:8157683-Chromosomes, Human, Pair 15, pubmed-meshheading:8157683-Chromosomes, Human, Pair 8, pubmed-meshheading:8157683-Chromosomes, Human, Pair 9, pubmed-meshheading:8157683-Cysteine Endopeptidases, pubmed-meshheading:8157683-Endopeptidases, pubmed-meshheading:8157683-Exons, pubmed-meshheading:8157683-Genomic Library, pubmed-meshheading:8157683-Hominidae, pubmed-meshheading:8157683-Humans, pubmed-meshheading:8157683-Introns, pubmed-meshheading:8157683-Karyotyping, pubmed-meshheading:8157683-Lung, pubmed-meshheading:8157683-Molecular Sequence Data, pubmed-meshheading:8157683-Organ Specificity, pubmed-meshheading:8157683-Promoter Regions, Genetic
pubmed:year
1994
pubmed:articleTitle
Human cathepsin S: chromosomal localization, gene structure, and tissue distribution.
pubmed:affiliation
Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't