Linked Life Data

815261

Source:http://linkedlifedata.com/resource/pubmed/id/815261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1976-6-2
pubmed:abstractText
Specific antiserum to a purified preparation of ornithine decarboxylase was prepared and successfully labeled with 125I. Monospecificity of this antiserum was attained by repeated precipitation with normal rat liver. The conditions for optimal antigen/antibody reaction were investigated. When the antibody was tested against either crude or purified regenerating rat liver enzyme a single precipitin line was observed both with Ouchterlony double diffusion plates and with immunoelectrophoresis. The specificity of the purified antiserum was also evaluated by sodium dodecyl sulfate gel electrophoresis of the 14C-labeled enzyme.antibody complex, the radioactivity of which appeared as a sharp peak in the 90,000 molecular weight region. Finally, an approximate antigen/antibody molar ratio was determined using labeled enzyme and labeled antiserum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
251
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1781-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Antiserum monospecific to hepatic ornithine decarboxylase.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.