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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1976-5-10
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pubmed:abstractText |
Polypeptide fragments corresponding to the NH2-terminal 55 amino acids of betaS and betaA globins were prepared by cyanogen bromide treatment of globin and isolated by gel filtration on Sephadex G-50. Sheep were immunized with the isolated NH2-terminal fragments, and one of these sheep produced precipitating antibodies to the NH2-terminal fragment of betaS globin. These antibodies also reacted with betaA and betaS globin and hemoglobins A and S, as shown by immunodiffusion and quantitative precipitation studies. A radioimmunoassay was developed using the radioiodinated NH2-terminal fragment as tracer, and dextran-coated charcoal for separating bound and free peptide. The radioimmunoassay was used to characterize the interaction of the antibodies and the NH2-terminal fragment of betaS globin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
251
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1283-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:815253-Amino Acids,
pubmed-meshheading:815253-Animals,
pubmed-meshheading:815253-Antibodies,
pubmed-meshheading:815253-Binding Sites,
pubmed-meshheading:815253-Binding Sites, Antibody,
pubmed-meshheading:815253-Globins,
pubmed-meshheading:815253-Hemoglobin, Sickle,
pubmed-meshheading:815253-Hemoglobins, Abnormal,
pubmed-meshheading:815253-Humans,
pubmed-meshheading:815253-Immunodiffusion,
pubmed-meshheading:815253-Kinetics,
pubmed-meshheading:815253-Precipitin Tests,
pubmed-meshheading:815253-Protein Binding,
pubmed-meshheading:815253-Sheep
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pubmed:year |
1976
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pubmed:articleTitle |
Antibodies to an NH2-terminal fragment of betaS globin. I. Preparation and radioimmunoassay.
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pubmed:publicationType |
Journal Article
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