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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1976-6-2
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pubmed:abstractText |
The lesions induced in Bacillus subtilis deoxyribonucleic acid (DNA) after treating bacterial cells (in vivo) and bacterial DNA (in vitro) with chloramine were studied biologically and physically. Single-strand breaks and a few double-strand scissions (at higher chloramine doses) accompanied loss of DNA-transforming activity in both kinds of treatments. Chloramine was about three times more efficient in vitro than in vivo in inducing DNA single-strand breaks. DNA was slowly chlorinated; the subsequent efficiency of producing DNA breaks was high. Chlorination of cells also reduced activity of endonucleases in cells; however, chlorinated DNA of both treatments was sensitized to cleavage by endonucleases. The procedure of extracting DNA from cells treated with chloramine induced further DNA degradation. Both treatments introduced a small fraction of alkali-sensitive lesions in DNA. DNA chlorinated in vitro showed further reduction in transforming activity as well as further degradation after incubation at 50 C for 5 h whereas DNA extracted from chloramine-treated cells did not show such a heat sensitivity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-13479462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-13670908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-13978795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-14016998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-14290352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-16590310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4298922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4567088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4633907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4636652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4938484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4954061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4961186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-4977986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/815252-5137279
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
934-45
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1976
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pubmed:articleTitle |
Effects of chloramine on Bacillus subtilis deoxyribonucleic acid.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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