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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1994-5-12
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pubmed:abstractText |
The simian virus 40 (SV40) large-T antigen is essential for SV40 DNA replication and for late viral gene expression, but the role of the SV40 small-t antigen in these processes is still unclear. We have previously demonstrated that small t inhibits SV40 DNA replication in vitro. In this study, we investigated the effect of small t on SV40 replication in cultured cells. CV1 monkey cell infection experiments indicated that mutant viruses that lack small t replicate less efficiently than the wild-type virus. We next microinjected CV1 cells with SV40 DNA with and without purified small-t protein and analyzed viral DNA replication efficiency by Southern blotting. Replication of either wild-type SV40 or small-t deletion mutant DNA was increased three- to fivefold in cells coinjected with purified small t. Thus, in contrast to our in vitro observation, small t stimulated viral DNA replication in vivo. This result suggests that small t has cellular effects that are not detectable in a reconstituted in vitro replication system. We also found that small t stimulated progression of permissive monkey cells--but not of nonpermissive rodent cells--from G0-G1 to the S phase of the cell cycle, possibly leading to an optimal intracellular environment for viral replication.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1310750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1310775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1310784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1316611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1529547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1570154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1649337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1658359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1663787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1706474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-174105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-178902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1847332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1847465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1848668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-1943760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-206892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-208777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-209467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-214244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2153055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2153460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2157202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2157892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2160857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2173140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-225037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-225524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2542592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2555176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2557059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2822947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-2996219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-3026642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-3034573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-4291934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6096557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6245271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6258954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6261020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6280457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6292479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6310338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6321781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-6323762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-659508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-8252625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8151779-8388518
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3138-44
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8151779-Animals,
pubmed-meshheading:8151779-Antigens, Viral, Tumor,
pubmed-meshheading:8151779-Cell Cycle,
pubmed-meshheading:8151779-Cells, Cultured,
pubmed-meshheading:8151779-Cercopithecus aethiops,
pubmed-meshheading:8151779-DNA, Viral,
pubmed-meshheading:8151779-DNA Replication,
pubmed-meshheading:8151779-Mice,
pubmed-meshheading:8151779-Microinjections,
pubmed-meshheading:8151779-Mutation,
pubmed-meshheading:8151779-Phosphorylation,
pubmed-meshheading:8151779-Protein Kinases,
pubmed-meshheading:8151779-Simian virus 40,
pubmed-meshheading:8151779-Virus Replication
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pubmed:year |
1994
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pubmed:articleTitle |
Simian virus 40 small-t antigen stimulates viral DNA replication in permissive monkey cells.
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pubmed:affiliation |
Section on DNA Replication, Repair, and Mutagenesis, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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