Linked Life Data

8151739

Source:http://linkedlifedata.com/resource/pubmed/id/8151739

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-5-6
pubmed:abstractText
In this study, the anti-idiotypic and anti-isotypic antibodies (McAbs) against IgM of the Patient with B chronic lymphocytic leukemia (B-CLL) were purified from hybridoma ascites by n-Octoic acid precipitation method. The purified McAbs have high purify and high antibody activity as evidenced by immunoelectrophoresis, SDS-PAGE and ELISA. Relative affinity of 11 McAbs was measured by using indirect ELISA and double antibody sandwich ELISA method. It was found that relative affinity of various McAbs to the same antigen was different. 11 McAbs could be divided into two groups by analysing their 50% maximum binding. The relative affinity of 4 McAbs in the culture supernatants was consistent with that of McAbs in the purified ascites. Our experimental results provide an important basis for rational application of these McAbs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0257-716X
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-7
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: VI. Purification and relative affinity of monoclonal antibodies.
pubmed:affiliation
Department of Immunology, Tongji Medical University, Wuhan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't