Linked Life Data

8150200

Source:http://linkedlifedata.com/resource/pubmed/id/8150200

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-5-9
pubmed:databankReference
pubmed:abstractText
We have isolated a cDNA of the DEAD protein family from a Xenopus ovary cDNA library. Northern hybridization revealed that the mRNA corresponding to the cDNA [XVLG1 (Xenopus vasa-like gene)] was specifically expressed in adult testis and ovary. The deduced amino acid sequence of XVLG1 was relatively homologous to that of Drosophila vasa. XVLG1 protein was found to be expressed exclusively in adult testis and ovary by immunoblotting with a monoclonal antibody against XVLG1 protein. Immunocytological study showed that XVLG1 protein was expressed in oogonia, oocytes, spermatogonia, spermatocytes, and also in primordial germ cells in stage 46 tadpoles. The monoclonal antibody used in this study specifically stains germ line cells and is useful both as a germ cell lineage marker and in biochemical studies of germ cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
354-63
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Isolation and characterization of a novel gene of the DEAD box protein family which is specifically expressed in germ cells of Xenopus laevis.
pubmed:affiliation
Furusawa Morphogene Project, ERATO, Research Development Corporation of Japan (JRDC), Tokyo, Japan.
pubmed:publicationType
Journal Article