|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0032000,
umls-concept:C0033684,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0439849,
umls-concept:C0445223,
umls-concept:C1456395,
umls-concept:C1552599,
umls-concept:C1704787,
umls-concept:C1880371,
umls-concept:C2911684
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1994-5-6
|
|
pubmed:abstractText |
Pit-1, a member of the POU family of homeo-domain transcription factors, activates prolactin and GH gene expression but also has a role in pituitary cell differentiation and proliferation. Expression of Pit-1 may therefore be of central importance in the function and phenotype of human pituitary adenomas. We have found evidence that, in addition to Pit-1 mRNA, Pit-1-like immunoreactivity and DNA-binding activity are readily detectable in a series of human pituitary adenomas. Gel mobility shift assays using adenoma protein extracts with two Pit-1-binding sites from the human prolactin gene promoter demonstrated the formation of several DNA sequence-specific protein-DNA complexes; some of these could be accounted for by Oct-1-binding activity. Pit-1 activity was anticipated in prolactin- and GH-secreting adenomas, but was also detected in a proportion of endocrine-inactive (non-secreting) adenomas that did not express Pit-1 target genes. The data demonstrate the presence of Pit-1 in a range of pituitary adenomas. Different adenomas generated slightly differing patterns of DNA-binding activity, though Pit-1 mRNA and protein size appeared normal in all tumours so far examined.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/POU1F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Pit-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0952-5041
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
283-90
|
|
pubmed:dateRevised |
2009-9-29
|
|
pubmed:meshHeading |
pubmed-meshheading:8148036-Adenoma,
pubmed-meshheading:8148036-Adult,
pubmed-meshheading:8148036-Aged,
pubmed-meshheading:8148036-Base Sequence,
pubmed-meshheading:8148036-DNA, Neoplasm,
pubmed-meshheading:8148036-DNA Primers,
pubmed-meshheading:8148036-DNA-Binding Proteins,
pubmed-meshheading:8148036-Female,
pubmed-meshheading:8148036-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8148036-Humans,
pubmed-meshheading:8148036-Immunohistochemistry,
pubmed-meshheading:8148036-Male,
pubmed-meshheading:8148036-Middle Aged,
pubmed-meshheading:8148036-Molecular Sequence Data,
pubmed-meshheading:8148036-Pituitary Neoplasms,
pubmed-meshheading:8148036-Protein Binding,
pubmed-meshheading:8148036-RNA, Messenger,
pubmed-meshheading:8148036-RNA, Neoplasm,
pubmed-meshheading:8148036-Transcription Factor Pit-1,
pubmed-meshheading:8148036-Transcription Factors
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Expression of Pit-1 and related proteins in diverse human pituitary adenomas.
|
|
pubmed:affiliation |
Department of Medicine, University of Manchester, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
