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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Suppl
|
| pubmed:dateCreated |
1994-5-5
|
| pubmed:abstractText |
We have monitored the end points of cellular survival, micronucleus formation and neoplastic transformation frequency to assess adaptation to ionizing radiation in the C3H 10T1/2 mouse embryo cell system. Plateau-phase cells were pre-exposed to an adapting dose of 0.1 to 1.5 Gy low-dose-rate gamma radiation 3.5 h prior to an acute challenge dose of 4 Gy. No adapting dose improved clonogenic survival detectably, whether the cells were plated immediately after the acute exposure or held in plateau phase for 3.5 h before plating. However, all chronic adapting doses resulted in both a reduction in micronucleus frequency in binucleate cells and about a twofold reduction in neoplastic transformation frequency per viable cell when cells were subsequently exposed to the 4-Gy challenge dose. Our data suggest that a low-dose-rate pre-exposure to ionizing radiation induces an adaptive response in C3H 10T1/2 cells, and that this response enhances DNA double-strand break repair when cells are subsequently exposed to a second radiation dose. This enhanced repair appears to be error-free since these adapted cells are also less susceptible to radiation-induced neoplastic transformation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0033-7587
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
138
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
S28-31
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8146320-Adaptation, Physiological,
pubmed-meshheading:8146320-Animals,
pubmed-meshheading:8146320-Cell Survival,
pubmed-meshheading:8146320-Cell Transformation, Neoplastic,
pubmed-meshheading:8146320-Cells, Cultured,
pubmed-meshheading:8146320-Embryo, Mammalian,
pubmed-meshheading:8146320-Gamma Rays,
pubmed-meshheading:8146320-Mice,
pubmed-meshheading:8146320-Mice, Inbred C3H,
pubmed-meshheading:8146320-Micronuclei, Chromosome-Defective,
pubmed-meshheading:8146320-Radiation Tolerance
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Radiation-induced adaptive response for protection against micronucleus formation and neoplastic transformation in C3H 10T1/2 mouse embryo cells.
|
| pubmed:affiliation |
Radiation Biology Branch, Chalk River Laboratories, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|