8144937

Source:http://linkedlifedata.com/resource/pubmed/id/8144937

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0379885, umls-concept:C0379886, umls-concept:C0597357, umls-concept:C1160185, umls-concept:C1337092, umls-concept:C1522558, umls-concept:C1704259, umls-concept:C1705079, umls-concept:C1705241, umls-concept:C1705242, umls-concept:C1705987, umls-concept:C2348205
pubmed:issue	7
pubmed:dateCreated	1994-4-29
pubmed:abstractText	MCP-2 and MCP-3 are recently identified members of the Cys-Cys chemokine family with high sequence similarity with MCP-1 (62% and 71%, respectively). The present study was aimed at defining receptor usage and signal transduction pathways of MCP-2 and MCP-3 in human monocytes in comparison with MCP-1. MCP-2 and MCP-3 induced migration of monocytes with a typical bell-shaped curve and maximal response at 10 and 50 ng/ml, respectively. The maximal response elicited by MCP-2 and MCP-3 was lower (approximately 60%) than that of MCP-1. Pertussis toxin (PTox) inhibited the chemotactic activity of MCP-3 and MCP-1 (IC50 = 6.2 and 4.4 ng/ml, respectively), whereas cholera toxin (CTox) had little effect on these two chemokines (IC50 > 1000 ng/ml). In contrast, MCP-2-induced chemotaxis was blocked by CTox (IC50 = 75 ng/ml) and relatively unaffected by PTox. MCP-3 and MCP-1 induced a rapid increase in intracellular Ca2+ concentration, whereas MCP-2, in the range of concentrations active on chemotaxis, did not. MCP-1-, MCP-2-, and MCP-3-induced chemotactic responses were blocked by C-I, a serine/threonine kinase inhibitor, and by genistein, a tyrosine kinase inhibitor, with the MCP-2 response being more sensitive than those induced by MCP-1 and MCP-3. MCP-1 and MCP-3 rapidly induced arachidonic acid release whereas MCP-2 was ineffective. MCP-1 and MCP-3 cross-desensitized with each other in terms of Ca2+ transients and displaced with a comparable efficiency labeled MCP-1 from human monocytes. On the other hand, MCP-2 did not cross-desensitize with MCP-1 and MCP-3 and only partially (20%) displaced labeled MCP-1. Thus, in spite of high sequence similarity, MCP-2 differed considerably from MCP-1 and MCP-3 in terms of sensitivity to CTox and PTox, arachidonate and calcium mobilization, and capacity to compete for labeled MCP-1.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2985117R
pubmed:citationSubset	AIM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid, http://linkedlifedata.com/resource/pubmed/chemical/CCL7 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CCL8 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL7, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL8, http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors, http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Cytokines, http://linkedlifedata.com/resource/pubmed/chemical/Monocyte Chemoattractant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR2, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine, http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
pubmed:status	MEDLINE
pubmed:month	Apr
pubmed:issn	0022-1767
pubmed:author	pubmed-author:LocatiMM, pubmed-author:MagazinMM, pubmed-author:MantovaniAA, pubmed-author:ProostPP, pubmed-author:RieppiMM, pubmed-author:SozzaniSS, pubmed-author:VitaNN, pubmed-author:ZhouDD, pubmed-author:van DammeJJ
pubmed:issnType	Print
pubmed:day	1
pubmed:volume	152
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3615-22
pubmed:dateRevised	2007-11-19
pubmed:meshHeading	pubmed-meshheading:8144937-Arachidonic Acid, pubmed-meshheading:8144937-Binding, Competitive, pubmed-meshheading:8144937-Calcium, pubmed-meshheading:8144937-Chemokine CCL2, pubmed-meshheading:8144937-Chemokine CCL7, pubmed-meshheading:8144937-Chemokine CCL8, pubmed-meshheading:8144937-Chemotactic Factors, pubmed-meshheading:8144937-Chemotaxis, Leukocyte, pubmed-meshheading:8144937-Cholera Toxin, pubmed-meshheading:8144937-Cytokines, pubmed-meshheading:8144937-Humans, pubmed-meshheading:8144937-Monocyte Chemoattractant Proteins, pubmed-meshheading:8144937-Monocytes, pubmed-meshheading:8144937-Pertussis Toxin, pubmed-meshheading:8144937-Receptors, CCR2, pubmed-meshheading:8144937-Receptors, Chemokine, pubmed-meshheading:8144937-Receptors, Cytokine, pubmed-meshheading:8144937-Second Messenger Systems, pubmed-meshheading:8144937-Signal Transduction, pubmed-meshheading:8144937-Virulence Factors, Bordetella
pubmed:year	1994
pubmed:articleTitle	Receptors and transduction pathways for monocyte chemotactic protein-2 and monocyte chemotactic protein-3. Similarities and differences with MCP-1.
pubmed:affiliation	Institute of Pharmacological Research Mario Negri, Milan, Italy.
pubmed:publicationType	Journal Article, In Vitro, Research Support, Non-U.S. Gov't