Linked Life Data

8143874

Source:http://linkedlifedata.com/resource/pubmed/id/8143874

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-5-5
pubmed:databankReference
pubmed:abstractText
The venom of the scorpion, Tityus serrulatus, was fractionated to investigate the chemical and pharmacological properties of its alpha-toxin content. Three alpha-toxins (Ts III, Ts IV and Ts V) were purified by conventional chromatography (gel filtration and ion-exchange chromatography), followed by immunoaffinity chromatography. Competition experiments using reference alpha- and beta-toxins suggested that these alpha-toxins were contaminated with around 0.01% of beta-toxin. The sequence of the first 30 amino acids of Ts IV was established. Using an oligonucleotide probe, a cDNA encoding its precursor was cloned from a venom gland cDNA library. The primary structure deduced from the cDNA nucleotide sequence provides possible explanations for the polymorphism of these three molecules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
342
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Biochemical, pharmacological and genomic characterisation of Ts IV, an alpha-toxin from the venom of the South American scorpion Tityus serrulatus.
pubmed:affiliation
Laboratoire de Biochimie, CNRS URA 1455, Faculté de Médecine secteur Nord, Marseille, France.
pubmed:publicationType
Journal Article, In Vitro