Linked Life Data

8141921

Source:http://linkedlifedata.com/resource/pubmed/id/8141921

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-4-29
pubmed:abstractText
In the present study, ethanol (Et-OH) and acetaldehyde (Ac-CHO) metabolism in primary cultured hepatocytes isolated from chronically alcohol-fed rats were analysed to elucidate the characteristics of the metabolism of Ac-CHO produced through the non-alcohol dehydrogenase (non-ADH) pathway. Dimethyl sulfoxide was added to the culture medium to prevent a decrease in P450 IIE1 activity. Ac-CHO formed in the culture medium was trapped by semicarbazide to prevent a reutilization of Ac-CHO by hepatocytes. The degradation rate of Ac-CHO in the liver was similar in alcohol and non-alcohol-treated rats. Therefore, the characteristic of the metabolism of Ac-CHO in the chronically alcohol-fed rats was not clarified in this study. The possibility that the Ac-CHO trapping by semicarbazide was only 70%, and that the remaining Ac-CHO was reutilized for oxidation in the hepatocytes, should be considered. Some method to trap Ac-CHO completely in the culture medium is needed to clarify the characteristics of Ac-CHO metabolism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1358-6173
pubmed:author
pubmed:issnType
Print
pubmed:volume
1A
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3-10
pubmed:dateRevised
2008-2-26
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Ethanol and acetaldehyde metabolism in cultured rat hepatocytes.
pubmed:affiliation
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
pubmed:publicationType
Journal Article, Comparative Study