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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-4-22
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pubmed:databankReference |
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pubmed:abstractText |
We have determined the nucleotide sequence (EMBL accession number, Z 21957) of the cloned chromosomal PER-1 extended-spectrum beta-lactamase gene from a Pseudomonas aeruginosa RNL-1 clinical isolate, blaPER-1 corresponds to a 924-bp open reading frame which encodes a polypeptide of 308 amino acids. This open reading frame is preceded by a -10 and a -35 region consistent with a putative P. aeruginosa promoter. Primer extension analysis of the PER-1 mRNA start revealed that this promoter was active in P. aeruginosa but not in Escherichia coli, in which PER-1 expression was driven by vector promoter sequences. N-terminal sequencing identified the PER-1 26-amino-acid leader peptide and enabled us to calculate the molecular mass (30.8 kDa) of the PER-1 mature form. Analysis of the percent GC content of blaPER-1 and of its 5' upstream sequences, as well as the codon usage for blaPER-1, indicated that blaPER-1 may have been inserted into P. aeruginosa genomic DNA from a nonpseudomonad bacterium. The PER-1 gene showed very low homology with other beta-lactamase genes at the DNA level. By using computer methods, assessment of the extent of identity between PER-1 and 10 beta-lactamase amino acid sequences indicated that PER-1 is a class A beta-lactamase. PER-1 shares around 27% amino acid identity with the sequenced extended-spectrum beta-lactamases of the TEM-SHV series and MEN-1 from Enterobacteriaceae species. The use of parsimony methods showed that PER-1 is not more closely related to gram-negative than to gram-positive bacterial class A beta-lactamases. Surprisingly, among class A beta-lactamases, PER-1 was most closely related to the recently reported CFXA from Bacteroides vulgatus, with which it shared 40% amino acid identity. This work indicates that non-Enterobacteriaceae species such as P. aeruginosa may possess class A extended-spectrum beta-lactamase genes possibly resulting from intergeneric DNA transfer.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8141562-1416873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8141562-1436034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8141562-149110,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8141562-8215276,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8141562-94251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:geneSymbol |
bla PER-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
104-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8141562-Amino Acid Sequence,
pubmed-meshheading:8141562-Base Sequence,
pubmed-meshheading:8141562-Cloning, Molecular,
pubmed-meshheading:8141562-Culture Media,
pubmed-meshheading:8141562-DNA, Bacterial,
pubmed-meshheading:8141562-Escherichia coli,
pubmed-meshheading:8141562-Molecular Sequence Data,
pubmed-meshheading:8141562-Molecular Weight,
pubmed-meshheading:8141562-Open Reading Frames,
pubmed-meshheading:8141562-Plasmids,
pubmed-meshheading:8141562-Pseudomonas aeruginosa,
pubmed-meshheading:8141562-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8141562-beta-Lactamases
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pubmed:year |
1994
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pubmed:articleTitle |
Sequence analysis of PER-1 extended-spectrum beta-lactamase from Pseudomonas aeruginosa and comparison with class A beta-lactamases.
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pubmed:affiliation |
Service de Microbiologie, Hôpital Raymond Poincaré, Faculté de Médecine Paris-Ouest, Université Paris, Garches, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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