Linked Life Data

8140729

Source:http://linkedlifedata.com/resource/pubmed/id/8140729

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-4-28
pubmed:abstractText
Eight day-old male and female ringneck pheasants (Phasianus colchicus) were inoculated with group D rotavirus and necropsied at 4, 7, and 11 days post-inoculation. The intestinal tracts were examined by light and electron microscopic and immunohistochemical methods. By 4 days post-inoculation, 2/3 (66%) inoculated birds were stunted and had diarrhea and dilated intestines. Intestinal villi were shortened, and many villous enterocytes were partially detached from the lamina propria. Crypts were hyperplastic, and the lamina propria contained a diffuse infiltrate of lymphocytes, plasma cells, and macrophages. Immunoreactivity to rotaviral antigen was localized to enterocytes on the tips of villi in the duodenum, jejunum, and proximal ileum. By 7 days post-inoculation, 3/3 (100%) inoculated birds had clinical signs and gross and microscopic changes similar to those at 4 days post-inoculation but more severe. Immunoreactivity was localized in enterocytes scattered along the sides of villi, in occasional crypt enterocytes, and within macrophages in the villous lamina propria. Ultrastructurally, infected enterocytes contained cytoplasmic aggregates of viroplasm with multiple viral core particles. Numerous mature virions (60-75 nm in diameter) were present within dilated components of the cytocavitary network. Macrophages within the lamina propria contained phagocytosed remnants of necrotic virus-infected cells. By 11 days post-inoculation, birds did not have gross lesions, but 1/2 (50%) had mild crypt hyperplasia and an infiltrate of leukocytes in the lamina propria. Occasional enterocytes along the sides of villi and macrophages in the lamina propria were immunoreactive for viral antigen.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0300-9858
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
74-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Morphogenesis of enteric lesions induced by group D rotavirus in ringneck pheasant chicks (Phasianus colchicus).
pubmed:affiliation
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't