Linked Life Data

8139446

Source:http://linkedlifedata.com/resource/pubmed/id/8139446

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-4-25
pubmed:abstractText
Myocardial lipid and lactate levels are sensitive indicators of biochemical status: lipid levels have been shown to increase in response to high fat diets, disease or metabolic stress and elevated lactate levels are indicative of reduced oxygen supply. Selective measurement of lactate or lipid levels by 1H NMR is not straightforward since both the lactate methyl group and lipid methylene groups resonate at 1.3 ppm. We have overcome this difficulty by employing spectral editing techniques to observe both lipid methylene and lactate methyl resonances, and have measured lipid and lactate levels in perfused rat hearts during control perfusion and in response to metabolic stress. Lactate increased during ischemia and decreased during reperfusion, and the ischemia-induced increase is inhibited by iodoacetate, as expected. In contrast, lipid levels increased during ischemia and remained elevated during reperfusion. Hearts from rats fed high fat diet show elevated lipid levels during control perfusion. Data obtained by 1H NMR are consistent with biochemical data, validating the technique.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0740-3194
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
655-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Simultaneous measurement of both lipid and lactate in isolated rat hearts by 1H NMR spectroscopy.
pubmed:affiliation
Institute for Biodiagnostics, National Research Council, Ottawa, Ontario, Canada.
pubmed:publicationType
Journal Article, In Vitro