|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1994-4-25
|
|
pubmed:abstractText |
Yeast mutants unable to grow in the presence of 3% formamide have been isolated in parallel with mutants sensitive to either 37 degrees or 6% ethanol. The number of formamide-sensitive mutations that affect different genes that can be identified from yeast cells is at least as large as the number of thermosensitive or ethanol-sensitive mutations. These mutations are of two types: those that are sensitive to formamide, temperature and/or ethanol simultaneously; and those that are specific for formamide sensitivity and show no temperature or ethanol sensitivity phenotype. Those genes susceptible to giving rise to formamide-sensitive alleles include the structural gene for DNA ligase, CDC9, and the structural gene for arginine permease, CAN1. The results indicate that formamide sensitivity can be used as a novel conditional phenotype for mutations on both essential and nonessential genes. This work also confirms that ethanol-sensitivity can be used as a conditional phenotype to identify mutations in at least as many genes as those susceptible to temperature or formamide sensitive mutations.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems, Basic,
http://linkedlifedata.com/resource/pubmed/chemical/CAN1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA ligase (ATP),
http://linkedlifedata.com/resource/pubmed/chemical/Ethanol,
http://linkedlifedata.com/resource/pubmed/chemical/Formamides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/arginine permease,
http://linkedlifedata.com/resource/pubmed/chemical/formamide
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0016-6731
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
136
|
|
pubmed:geneSymbol |
CAN1,
CDC9
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
87-91
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8138179-Alleles,
pubmed-meshheading:8138179-Amino Acid Transport Systems,
pubmed-meshheading:8138179-Amino Acid Transport Systems, Basic,
pubmed-meshheading:8138179-Crosses, Genetic,
pubmed-meshheading:8138179-DNA Ligases,
pubmed-meshheading:8138179-Ethanol,
pubmed-meshheading:8138179-Formamides,
pubmed-meshheading:8138179-Genes, Fungal,
pubmed-meshheading:8138179-Hot Temperature,
pubmed-meshheading:8138179-Membrane Transport Proteins,
pubmed-meshheading:8138179-Mutagenesis,
pubmed-meshheading:8138179-Phenotype,
pubmed-meshheading:8138179-Saccharomyces cerevisiae,
pubmed-meshheading:8138179-Saccharomyces cerevisiae Proteins
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Formamide sensitivity: a novel conditional phenotype in yeast.
|
|
pubmed:affiliation |
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
