Linked Life Data

8138158

Source:http://linkedlifedata.com/resource/pubmed/id/8138158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-4-25
pubmed:abstractText
Segregation Distorter (SD) is a naturally occurring system of meiotic drive in Drosophila melanogaster. Males heterozygous for an SD second chromosome and a normal homolog (SD+) transmit predominantly SD-bearing sperm. To accomplish this, the Segregation distorter (Sd) locus induces the dysfunction of those spermatids that receive the SD+ chromosome. Recently, P. A. Powers and B. Ganetzky isolated overlapping DNA clones spanning the region of DNA known to contain the Sd gene and identified a 5-kb tandem duplication that is present on all SD chromosomes examined, but is apparently absent from wild-type chromosomes. Here we report a molecular analysis of two spontaneous revertants from an Australian SD chromosome (SD-Arm28). Both of these revertants have lost the 5-kb tandem duplication along with the ability to distort transmission; the critical observation, however, is that they retain the DNA haplotype in the flanking regions (both proximally and distally) that is characteristic of the original SD-Arm28. We propose unequal sister chromatid exchange between the tandem repeats as the only plausible explanation for loss of a repeat while retaining flanking markers. This provides direct evidence that the tandem duplication is indeed necessary for the Sd phenotype. Further, we examined testes-specific levels of both RNA and protein for the nearby Topoisomerase 2 gene. Neither revealed a consistent difference between SD and SD+ strains. We also measured testes-specific levels of RNA using the tandem duplication itself as probe. Our results suggest that there is strong up-regulation of one or several 2.0-2.3-kb transcripts from the duplicated region in the testes of an SD strain.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-1722553, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-17246219, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-17249048, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-1906417, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-1936954, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-2124974, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-2441880, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-2498161, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-2538621, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-2839299, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-401895, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-407128, http://linkedlifedata.com/resource/pubmed/commentcorrection/8138158-4197166
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-15
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Characterization of two Segregation distorter revertants: evidence that the tandem duplication is necessary for Sd activity in Drosophila melanogaster.
pubmed:affiliation
Department of Ecology and Evolution, University of Chicago, Illinois 60637.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't