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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
1994-4-26
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pubmed:abstractText |
We investigated the role of the 1st cytoplasmic loop of the thyrotropin receptor (TSHR) on signal transduction using mutants as we did the 2nd and 3rd cytoplasmic loops [Kosugi S. et al. (1994) Mol. Endocrinol., in press; and 7, 1009-1020]. Five substitution mutants involving the first cytoplasmic loop showed a TSH- or Graves' IgG-stimulated cAMP response despite the low TSH binding Bmax. All the mutants completely lost or markedly decreased the TSH- or Graves' IgG-stimulated inositol phosphate increase. These findings suggest that the 1st cytoplasmic loop of the TSHR does not play a crucial role in agonist-induced adenylate cyclase activation but that it is important for phosphoinositide signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
341
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
162-6
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pubmed:dateRevised |
2001-11-2
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pubmed:meshHeading |
pubmed-meshheading:8137933-Adenylate Cyclase,
pubmed-meshheading:8137933-Amino Acid Sequence,
pubmed-meshheading:8137933-Cell Line,
pubmed-meshheading:8137933-Cyclic AMP,
pubmed-meshheading:8137933-Cytoplasm,
pubmed-meshheading:8137933-Enzyme Activation,
pubmed-meshheading:8137933-Immunoglobulin G,
pubmed-meshheading:8137933-Molecular Sequence Data,
pubmed-meshheading:8137933-Mutagenesis, Site-Directed,
pubmed-meshheading:8137933-Phosphatidylinositols,
pubmed-meshheading:8137933-Receptors, Thyrotropin,
pubmed-meshheading:8137933-Signal Transduction,
pubmed-meshheading:8137933-Thyrotropin
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pubmed:year |
1994
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pubmed:articleTitle |
The first cytoplasmic loop of the thyrotropin receptor is important for phosphoinositide signaling but not for agonist-induced adenylate cyclase activation.
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pubmed:affiliation |
Department of Laboratory Medicine, Kyoto University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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