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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-4-25
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pubmed:abstractText |
The mammalian nuclear protein E2F-1 has recently been cloned based on its ability to bind the retinoblastoma protein. To determine whether E2F-1 plays a role in the control of the cell proliferation, we introduced an inducible construct expressing an E2F-1 antisense RNA into the human glioblastoma T98G cell line and assessed DNA synthesis during the cell cycle. Expression of the antisense transcripts during the G1-S transition resulted in a marked delay in the completion of DNA synthesis. Band-shift analysis of bacterially produced E2F-1 showed that this protein bound to the promoters of human DNA polymerase-alpha, cyclin D1, and c-myb but not to the cdc2 gene promoter. E2F-1 also transactivated the bound promoters in transient transfection assays. These results suggest a major role for E2F-1 in the control of cell cycle progression via transcriptional regulation of proliferation-associated genes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDC2 Protein Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E2F Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/E2F1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/E2F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein p55(v-myc),
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor DP1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
54
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pubmed:geneSymbol |
E2F-1,
RBAP-1,
cdc2,
pRB
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1402-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8137237-Base Sequence,
pubmed-meshheading:8137237-CDC2 Protein Kinase,
pubmed-meshheading:8137237-Carrier Proteins,
pubmed-meshheading:8137237-Cell Cycle,
pubmed-meshheading:8137237-Cell Cycle Proteins,
pubmed-meshheading:8137237-Cyclin D1,
pubmed-meshheading:8137237-Cyclins,
pubmed-meshheading:8137237-DNA, Antisense,
pubmed-meshheading:8137237-DNA, Complementary,
pubmed-meshheading:8137237-DNA, Neoplasm,
pubmed-meshheading:8137237-DNA Polymerase II,
pubmed-meshheading:8137237-DNA-Binding Proteins,
pubmed-meshheading:8137237-E2F Transcription Factors,
pubmed-meshheading:8137237-E2F1 Transcription Factor,
pubmed-meshheading:8137237-Glioblastoma,
pubmed-meshheading:8137237-Humans,
pubmed-meshheading:8137237-Molecular Sequence Data,
pubmed-meshheading:8137237-Oncogene Protein p55(v-myc),
pubmed-meshheading:8137237-Oncogene Proteins,
pubmed-meshheading:8137237-Promoter Regions, Genetic,
pubmed-meshheading:8137237-Retinoblastoma-Binding Protein 1,
pubmed-meshheading:8137237-S Phase,
pubmed-meshheading:8137237-Transcription, Genetic,
pubmed-meshheading:8137237-Transcription Factor DP1,
pubmed-meshheading:8137237-Transcription Factors,
pubmed-meshheading:8137237-Transcriptional Activation,
pubmed-meshheading:8137237-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Correlation between E2F-1 requirement in the S phase and E2F-1 transactivation of cell cycle-related genes in human cells.
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pubmed:affiliation |
Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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