Linked Life Data

8134624

Source:http://linkedlifedata.com/resource/pubmed/id/8134624

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-4-20
pubmed:abstractText
Serological tests for cowdriosis are hampered by cross-reacting antibodies from animals suspected to be infected with Ehrlichia species. We have monitored infections with Ehrlichia bovis, E. ovina, E. canis and E. phagocytophila in experimental animals by competitive ELISA, Western blotting and immunofluorescence using Cowdria-infected endothelial cell culture antigens. Cross-reactions due to Ehrlichia antibodies could be attributed to the recognition of epitopes on the immunodominant Cr32 Cowdria protein. This was especially true for E. canis and E. ovina, much less for E. bovis, but not at all for E. phagocytophila. In addition, strong cross-reactivity between Cowdria and antibodies to E. Chaffeenis were demonstrated. These findings are in agreement with the phylogenetic relationships, recently reported by van Vliet et al. in 1992, between Cowdria and other members of the tribe Ehrlichieae, which showed Cowdria to be closely related to E. canis and also to E. chaffeensis. Although the tests used in this study remain valuable tools under laboratory conditions, their specificity requires improvement. It is suggested to study recombinant Cowdria antigens for the development of second generation serological tests for cowdriosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0035-1865
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
The immunodominant 32-kilodalton protein of Cowdria ruminantium is conserved within the genus Ehrlichia.
pubmed:affiliation
Department of Parasitology and Tropical Veterinary Medicine, University of Utrecht, Pays-Bas.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't