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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-4-21
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pubmed:abstractText |
At weaning the mammary gland undergoes a reductive remodelling process (involution) which is associated with the cessation of milk protein gene expression and programmed cell death of milk-producing epithelial cells. Elevated nuclear protein kinase A (PKA) activity was observed from one day post-lactation, paralleled by increased c-fos, junB, junD and to a lesser extent c-jun mRNA levels. AP-1 DNA binding activity was transiently induced and the AP-1 complex was shown to consist principally of cFos/JunD. Oct-1 DNA binding activity and Oct-1 protein were gradually lost from the gland over the first 4 days of involution, whereas Oct-1 mRNA levels remained unchanged. Comparing nuclear extracts from normal mammary glands with nuclear extracts from glands which had been cleared of all epithelial cells 3 weeks after birth, revealed that PKA activation, AP-1 induction and Oct-1 inactivation all are dependent on the presence of the epithelial compartment. The increased Fos/Jun expression and the inactivation of Oct-1 may be consequences of the increased PKA activity. A similar induction of AP-1 (cFos/JunD) was also observed in the involuting rat ventral prostate pointing to a possible role for AP-1 in programmed cell death.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caseins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hcfc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Host Cell Factor C1,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/whey protein
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1213-23
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8134124-Animals,
pubmed-meshheading:8134124-Apoptosis,
pubmed-meshheading:8134124-Base Sequence,
pubmed-meshheading:8134124-Caseins,
pubmed-meshheading:8134124-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:8134124-DNA-Binding Proteins,
pubmed-meshheading:8134124-Down-Regulation,
pubmed-meshheading:8134124-Gene Expression Regulation,
pubmed-meshheading:8134124-Host Cell Factor C1,
pubmed-meshheading:8134124-Lactation,
pubmed-meshheading:8134124-Male,
pubmed-meshheading:8134124-Mammary Glands, Animal,
pubmed-meshheading:8134124-Mice,
pubmed-meshheading:8134124-Mice, Inbred BALB C,
pubmed-meshheading:8134124-Milk Proteins,
pubmed-meshheading:8134124-Molecular Sequence Data,
pubmed-meshheading:8134124-Octamer Transcription Factor-1,
pubmed-meshheading:8134124-Prostate,
pubmed-meshheading:8134124-Protein Binding,
pubmed-meshheading:8134124-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:8134124-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:8134124-Rats,
pubmed-meshheading:8134124-Rats, Wistar,
pubmed-meshheading:8134124-Transcription Factors,
pubmed-meshheading:8134124-Weaning
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pubmed:year |
1994
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pubmed:articleTitle |
Protein kinase A and AP-1 (c-Fos/JunD) are induced during apoptosis of mouse mammary epithelial cells.
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pubmed:affiliation |
Laboratory for Clinical and Experimental Research, University of Bern, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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