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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1994-4-21
|
| pubmed:abstractText |
Exogenous HGF/SF converts subconfluent cultures of NBT-II epithelial carcinoma cells into mobile fibroblast-like cells while being only mitogenic for cells maintained at high density. To investigate the potential role of such factor in tumor progression, we generated HGF/SF-producing NBT-II cells by transfection with an expression plasmid containing human HGF/SF cDNA. HGF/SF-producing cells also exhibit a fibroblastic phenotype. Media conditioned by these cells are potent inducers of in vitro tubulogenesis which can be inhibited with specific anti-HGF/SF antibodies; these antibodies are also able to reverse the scattered phenotype of the HGF/SF-producing cells. In addition spheroids of HGF/SF-producing cells are dispersed into 3D collagen gels suggesting an increase of invasive properties of these cells. When injected in nude mice, these HGF/SF-producing cells induce tumors appearing more rapidly than did those obtained with untransfected cells. These results show that HGF/SF can promote motility and invasive properties of NBT-II bladder carcinoma cells and also confers a tumorigenic advantage when acting as an autocrine factor.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1091-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8134112-Animals,
pubmed-meshheading:8134112-Antibody Specificity,
pubmed-meshheading:8134112-Cell Adhesion,
pubmed-meshheading:8134112-Cell Division,
pubmed-meshheading:8134112-Culture Media, Conditioned,
pubmed-meshheading:8134112-Female,
pubmed-meshheading:8134112-Fibroblasts,
pubmed-meshheading:8134112-Hepatocyte Growth Factor,
pubmed-meshheading:8134112-Humans,
pubmed-meshheading:8134112-Mice,
pubmed-meshheading:8134112-Mice, Nude,
pubmed-meshheading:8134112-Neoplasm Invasiveness,
pubmed-meshheading:8134112-Neoplasm Metastasis,
pubmed-meshheading:8134112-Neoplasms, Experimental,
pubmed-meshheading:8134112-Rats,
pubmed-meshheading:8134112-Transfection,
pubmed-meshheading:8134112-Tumor Cells, Cultured,
pubmed-meshheading:8134112-Urinary Bladder Neoplasms
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Creation of an hepatocyte growth factor/scatter factor autocrine loop in carcinoma cells induces invasive properties associated with increased tumorigenicity.
|
| pubmed:affiliation |
Laboratoire de Physiopathologie du Développement, URA 1337 CNRS, Ecole Normale Supérieure, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|