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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6470
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pubmed:dateCreated |
1994-4-19
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pubmed:abstractText |
Expression of antigen receptors is vital for the development of B and T lymphocytes. In mice with the scid mutation, which are unable to make productive rearrangements of their immunoglobulin and T-cell receptor (TCR) genes, lymphopoiesis aborts at an early stage. The death of the immature lymphocytes by apoptosis is postulated to result from a failure to receive a survival signal induced by receptor engagement. Consistent with this hypothesis, introduction of immunoglobulin or TCR transgenes into scid mice promoted an increase in B- or T-lymphoid cells, respectively. As the protein encoded by the bcl-2 gene can inhibit cell death, we tested whether lymphopoiesis could be rescued in scid mice by crossing in a bcl-2 transgene. Strikingly, the bcl-2/scid mice accumulated almost normal numbers of B-lymphoid cells which lacked surface immunoglobulin but expressed markers of maturity. T-cell development remained blocked. Introducing a TCR transgene enabled bcl-2/scid mice to develop normal numbers of CD4+8+ thymocytes even in the absence of immunological selection, suggesting that T cells become competent to respond to bcl-2 protein only after the TCR complex is displayed at the cell surface.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
368
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pubmed:geneSymbol |
bcl-2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
457-60
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8133891-Animals,
pubmed-meshheading:8133891-Antigens, Differentiation,
pubmed-meshheading:8133891-B-Lymphocytes,
pubmed-meshheading:8133891-Base Sequence,
pubmed-meshheading:8133891-Bone Marrow Cells,
pubmed-meshheading:8133891-Cell Differentiation,
pubmed-meshheading:8133891-Cell Survival,
pubmed-meshheading:8133891-Cells, Cultured,
pubmed-meshheading:8133891-DNA Primers,
pubmed-meshheading:8133891-Immunophenotyping,
pubmed-meshheading:8133891-Mice,
pubmed-meshheading:8133891-Mice, SCID,
pubmed-meshheading:8133891-Mice, Transgenic,
pubmed-meshheading:8133891-Molecular Sequence Data,
pubmed-meshheading:8133891-Proto-Oncogene Proteins,
pubmed-meshheading:8133891-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8133891-Receptors, Antigen, B-Cell,
pubmed-meshheading:8133891-Receptors, Antigen, T-Cell,
pubmed-meshheading:8133891-Spleen,
pubmed-meshheading:8133891-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
Bcl-2 expression promotes B- but not T-lymphoid development in scid mice.
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pubmed:affiliation |
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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