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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-4-21
|
| pubmed:abstractText |
The prevalence and genotype distribution of Actinobacillus actinomycetemcomitans strains in families where at least one adult family member (proband) suffered from periodontal disease was investigated to better understand how this periodontal organism is acquired or transmitted. Fifteen probands with severe (established) periodontal disease (EPD) and their 46 immediate family members were sampled for A. actinomycetemcomitans. Among the 15 families, 10 contained at least one additional family member colonized with oral A. actinomycetemcomitans. Genomic DNA from 3 subgingival A. actinomycetemcomitans strains from each of the 10 probands and their 17 family members were amplified and characterized by the polymerase chain reaction (PCR) using a single arbitrary primer known to distinguish A. actinomycetemcomitans strains. The PCR products from each strain were separated by electrophoresis on a 1% submarine agarose gel containing ethidium bromide and visualized by UV light transillumination. The amplification products migrated to form readily distinguishable bands and, since the banding patterns were characteristic of strains of A. actinomycetemcomitans, these patterns were called "amplitypes." The culture studies showed that 51% of all patients suffering from EPD carried oral A. actinomycetemcomitans. Moreover, 50% of their spouses and 30% of their children harbored the bacterium. Comparison of the PCR-generated amplitypes showed that 26 out of 27 individuals had strains exhibiting a single amplitype of A. actinomycetemcomitans, the 27th being colonized by 2 different amplitypes. They also showed that in 6 out of 7 families, the husband and wife did not harbor the same A. actinomycetemcomitans amplitype. Furthermore, most often children carried an an amplitype identical to one of the parents.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
D
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0022-3492
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8133411-Actinobacillus Infections,
pubmed-meshheading:8133411-Actinobacillus actinomycetemcomitans,
pubmed-meshheading:8133411-Adult,
pubmed-meshheading:8133411-Aged,
pubmed-meshheading:8133411-Bacterial Typing Techniques,
pubmed-meshheading:8133411-Base Sequence,
pubmed-meshheading:8133411-Child,
pubmed-meshheading:8133411-DNA, Bacterial,
pubmed-meshheading:8133411-DNA Fingerprinting,
pubmed-meshheading:8133411-DNA Primers,
pubmed-meshheading:8133411-Electrophoresis, Agar Gel,
pubmed-meshheading:8133411-Family Health,
pubmed-meshheading:8133411-Female,
pubmed-meshheading:8133411-Humans,
pubmed-meshheading:8133411-Male,
pubmed-meshheading:8133411-Middle Aged,
pubmed-meshheading:8133411-Molecular Sequence Data,
pubmed-meshheading:8133411-Periodontitis,
pubmed-meshheading:8133411-Polymerase Chain Reaction,
pubmed-meshheading:8133411-Prevalence
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
The distribution and transmission of Actinobacillus actinomycetemcomitans in families with established adult periodontitis.
|
| pubmed:affiliation |
Department of Periodontology, Faculty of Dentistry, Oslo, Norway.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|