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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-4-18
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pubmed:abstractText |
A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The tetradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibody was purified and used for a one-step immunodetection assay of non-radioactive DNA and RNA probes. The immunoassay developed was able to detect 5 pg DNA within 2 h and gave low background noise.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1759
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-33
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8133070-2-Acetylaminofluorene,
pubmed-meshheading:8133070-Alkaline Phosphatase,
pubmed-meshheading:8133070-Animals,
pubmed-meshheading:8133070-Antibodies, Bispecific,
pubmed-meshheading:8133070-Cell Line,
pubmed-meshheading:8133070-Cells, Cultured,
pubmed-meshheading:8133070-DNA,
pubmed-meshheading:8133070-DNA, Bacterial,
pubmed-meshheading:8133070-Electrophoresis, Agar Gel,
pubmed-meshheading:8133070-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8133070-Hybridomas,
pubmed-meshheading:8133070-Immunoblotting,
pubmed-meshheading:8133070-Immunoglobulin G,
pubmed-meshheading:8133070-Mice,
pubmed-meshheading:8133070-Mice, Inbred BALB C,
pubmed-meshheading:8133070-Nucleic Acid Hybridization,
pubmed-meshheading:8133070-Xanthomonas campestris
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pubmed:year |
1994
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pubmed:articleTitle |
Development of a bispecific monoclonal antibody for use in molecular hybridisation.
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pubmed:affiliation |
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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