Linked Life Data

8133031

Source:http://linkedlifedata.com/resource/pubmed/id/8133031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-4-19
pubmed:databankReference
pubmed:abstractText
The molecular basis for the surface expression of TCR-beta chain in the absence of association with TCR-alpha, -gamma, or -delta chain by an immature thymocyte cell line was investigated. The TCR-beta chain expressed by this cell line was not encoded by any unique DNA sequence, nor was it inserted into the membrane via a glycosyl-phosphatidylinositol linkage. Transfection of two other beta-chains derived from mature T cell clones resulted in the surface expression of dimers of the transfected beta-chains in both cases. Immunoprecipitation of the beta-dimer-CD3 complex demonstrated that the association of the beta-dimer with the CD3 complex, especially the CD3 zeta chain, was so weak that they dissociated under the detergent conditions in which the TCR-CD3 complex of mature T cells is kept intact. Transfection of TCR-alpha chain resulted in the expression of a TCR-alpha beta-CD3 complex and the disappearance of beta-dimers. In accordance with the changes in TCR complex components, the association between TCR-alpha beta and CD3 complex became stable and the cells transduced signals more efficiently. The results demonstrate that the expression of TCR-beta as part of an incomplete TCR-CD3 complex is developmentally regulated and the expression of TCR-alpha chain results in normal configuration and function of TCR complex.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2148-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8133031-Animals, pubmed-meshheading:8133031-Antigens, CD3, pubmed-meshheading:8133031-Base Sequence, pubmed-meshheading:8133031-Cell Differentiation, pubmed-meshheading:8133031-Cell Line, pubmed-meshheading:8133031-DNA, Complementary, pubmed-meshheading:8133031-DNA Primers, pubmed-meshheading:8133031-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, pubmed-meshheading:8133031-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, pubmed-meshheading:8133031-Glycosylphosphatidylinositols, pubmed-meshheading:8133031-Mice, pubmed-meshheading:8133031-Molecular Sequence Data, pubmed-meshheading:8133031-Protein Conformation, pubmed-meshheading:8133031-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:8133031-T-Lymphocytes, pubmed-meshheading:8133031-Transfection
pubmed:year
1994
pubmed:articleTitle
Transition from TCR-beta dimer to TCR-alpha beta-expressing cells by introduction of an alpha-chain in an immature thymocyte cell line.
pubmed:affiliation
Division of Molecular Genetics, Chiba University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't