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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1994-4-21
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pubmed:databankReference |
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pubmed:abstractText |
NaCl-sensitive yeast mutants were isolated to identify genes essential for NaCl tolerance. Complementation of a mutant highly sensitive to Na+ and Li+ led to the isolation of the CNB1 gene. This gene encodes the regulatory subunit (CNB) of the Ca2+/calmodulin-dependent protein phosphatase calcineurin. Cells deficient in CNB accumulated Li+ due to reduced expression of ENA1, a gene encoding a P-type ATPase involved in Na+ and Li+ efflux. In addition, the K+ transport system of cnb1 delta cells was not converted to the high affinity state that facilitates better discrimination of K+ over Na+. Thus the cnb1 delta strain resembled a trk1 mutant. These results indicate that adaptation to NaCl stress in Saccharomyces cerevisiae requires a signal transduction pathway involving Ca2+ and protein phosphorylation-dephosphorylation. In this pathway, calcineurin would coordinate gene expression and activity of ion transporters to facilitate ion homeostasis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Calcineurin,
http://linkedlifedata.com/resource/pubmed/chemical/Calmodulin-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cation Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lithium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
269
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pubmed:geneSymbol |
CNB1,
DPH2,
ENA1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8792-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8132612-Adenosine Triphosphatases,
pubmed-meshheading:8132612-Base Sequence,
pubmed-meshheading:8132612-Biological Transport, Active,
pubmed-meshheading:8132612-Calcineurin,
pubmed-meshheading:8132612-Calmodulin-Binding Proteins,
pubmed-meshheading:8132612-Cation Transport Proteins,
pubmed-meshheading:8132612-Chromosome Mapping,
pubmed-meshheading:8132612-Cloning, Molecular,
pubmed-meshheading:8132612-Fungal Proteins,
pubmed-meshheading:8132612-Genes, Fungal,
pubmed-meshheading:8132612-Genetic Complementation Test,
pubmed-meshheading:8132612-Homeostasis,
pubmed-meshheading:8132612-Lithium Chloride,
pubmed-meshheading:8132612-Molecular Sequence Data,
pubmed-meshheading:8132612-Phosphoprotein Phosphatases,
pubmed-meshheading:8132612-Restriction Mapping,
pubmed-meshheading:8132612-Saccharomyces cerevisiae,
pubmed-meshheading:8132612-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8132612-Sodium,
pubmed-meshheading:8132612-Sodium Chloride,
pubmed-meshheading:8132612-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:8132612-Water-Electrolyte Balance
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pubmed:year |
1994
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pubmed:articleTitle |
The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae.
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pubmed:affiliation |
Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas, Sevilla, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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