Linked Life Data

8131078

Source:http://linkedlifedata.com/resource/pubmed/id/8131078

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-4-15
pubmed:abstractText
The effect of chronic exposure to alcohol during development on the calcium currents of rat cerebellar Purkinje neurons was studied in a culture model system using voltage clamp techniques. The neurons were exposed to 30 mM alcohol (ethanol) during the main period of morphological and physiological development. The calcium currents were measured at the end of the treatment period, which lasted for 8-10 days. The currents were evoked by a series of depolarizing test commands from holding potentials of -62 mV and -90 mV. The evoked currents were qualitatively similar in control and alcohol-treated neurons and were comprised of a high threshold slowly inactivating calcium current and a low threshold rapidly inactivating calcium current. The low threshold current could be observed in isolation at test potentials ranging from -50 to -30 mV. The mean peak amplitude of this current was significantly smaller in the alcohol-treated neurons compared to controls. At more depolarized test potentials, the high threshold current dominated the current response, which was characterized by an initial peak that slowly declined to a smaller relatively sustained level. The mean amplitude of the high threshold current at both peak and sustained levels was significantly larger in the alcohol-treated neurons compared to controls. Measurement of cell size indicated that alcohol-treated neurons were approximately 25% smaller than control neurons, a difference that could contribute to the smaller low threshold current observed in these neurons. These data show that chronic exposure to alcohol during the development can significantly influence the amplitude of calcium currents of the cultured Purkinje neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
634
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Chronic exposure to alcohol during development alters the calcium currents of cultured cerebellar Purkinje neurons.
pubmed:affiliation
Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.