Linked Life Data

8130283

Source:http://linkedlifedata.com/resource/pubmed/id/8130283

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-4-15
pubmed:abstractText
This report describes a method for assay of choline (Ch) and acetylcholine (ACh) levels in rodent brain regions by high-performance liquid chromatography combined with continuous-flow fast atom bombardment mass spectrometry. Following an investigation of various extraction procedures and chromatographic conditions, we found that homogenization in acetonitrile in the presence of magnesium sulphate followed by heptane extraction and then a flow rate programme for chromatographic separation yielded the best results of the protocols tested. Under these conditions, the recoveries of Ch and ACh were 75% and 80%, respectively, with detection limits of 5 pmol for Ch and 2 pmol for ACh. Using this method, Ch and ACh levels in rat and mouse brain regions were similar to those reported by other researchers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1052-9306
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
681-6
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Use of high-performance liquid chromatography continuous-flow fast atom bombardment mass spectrometry for simultaneous determination of choline and acetylcholine in rodent brain regions.
pubmed:affiliation
Department of Neuropsychopharmacology (Tsumura), Gunma University School of Medicine, Japan.
pubmed:publicationType
Journal Article