Linked Life Data

8130202

Source:http://linkedlifedata.com/resource/pubmed/id/8130202

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1994-4-18
pubmed:abstractText
A variety of plant growth regulators belonging to the auxin phytohormone family have been found to be good competitive inhibitors of the oxynitrilase from almonds, mandelonitrile lyase (MNL). The major natural auxin, indole-3-acetic acid (IAA), was found to inactivate MNL in a reaction following pseudo-first-order kinetics and dependent upon visible light. Inactivation results from the oxidative decarboxylation of IAA forming 3-methyleneoxidole (MOI). This compound has been synthesized and shown to produce active-site-directed inactivation of MNL, in a reaction following saturation kinetics with a KI of 37 +/- 8 microM and maximal kinact of 0.13 +/- 0.02 min-1. Inactivation protection is provided by the competitive inhibitors azide and benzoate, suggesting that the inactivation reaction is active-site-directed. This idea is substantiated by our determination that MOI is a competitive inhibitor of MNL with a Ki of 23 +/- 3 microM under steady-state turnover conditions, in reasonable agreement with the value obtained from the inactivation data. Several indole derivatives such as indoline, skatole, oxindole, and 3-methyloxindole are poor competitive inhibitors of MNL with dissociation constants 20-40-fold greater than that for MOI, suggesting a highly specific binding site for the IAA photooxidation product. The enzyme remains inactive following spin dialysis, indicating that a covalent adduct has been formed. However, approximately 30% activity was recovered in a 5-h period following dialysis, and a nearly quantitative recovery occurs in the presence of 2-mercaptoethanol or DTT, indicating that the adduct is labile.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2891-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Transient inactivation of almond mandelonitrile lyase by 3-methyleneoxindole: a photooxidation product of the natural plant hormone indole-3-acetic acid.
pubmed:affiliation
Department of Chemistry, Tufts University, Medford, Massachusetts 02155.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't