Linked Life Data

8128803

Source:http://linkedlifedata.com/resource/pubmed/id/8128803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9-10
pubmed:dateCreated
1994-4-12
pubmed:abstractText
Cell wall antigens from Coxiella burnetti were extracted by heat, ultrasonication, phenol, SDS or sodium deoxycholate. These antigens were subsequently purified by gel-chromatography yielding molecular weights of 700 and 270 KD. Using gel-chromatographical purification of antigens extracted by heat, ultrasonication or phenol Coxiella antigens nearly devoid of contaminations could be obtained. Of these highly purified antigens heat extracted antigen was treated with proteases, lysozyme or metaperiodate exemplarily. These treatments did neither destroy nor change its antigenic activity, except for the treatment with metaperiodate reducing the antigenic activity significantly. In further experiments heat extracted and gel-chromatographically purified Coxiella antigen was used for testing bovine sera in ELISA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0514-7166
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
690-6
pubmed:dateRevised
2008-2-13
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA).
pubmed:affiliation
Staatliches Tierärztliches Untersuchungsamt Stuttgart, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't