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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1994-4-12
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pubmed:abstractText |
Lysophosphatidic acid (LPA) is an important constituent of serum and shares its mitogenic activity. Serum induction of several genes is regulated, at least in part, by sequences related to the c-fos serum response element (SRE). A Rat-2 fibroblast cell line containing the beta-galactosidase reporter gene under SRE control was treated with LPA. Lysophosphatidic acid induced a time- and dose-dependent increase in beta-galactosidase activity. After 5 hours of treatment with 1-oleoyl-LPA a 3-fold increase in beta-galactosidase activity was observed. In contrast, endogenous alkaline phosphatase activity did not change in parallel with the beta-galactosidase activity indicating that the induction was specific. Various LPAs with different acyl groups in the sn-1 position induced beta-galactosidase activity with a rank order potency of 1-oleoyl-LPA > 1-palmitoyl-LPA > or = 1-myristoyl-LPA > 1-stearoyl-LPA. Phosphatidic acid was approximately equal to 1-stearoyl-LPA. Neither the calcium ionophore (A23187) nor 12-O-tetradecanoylphorbol 13-acetate, induced beta-galactosidase activity. These data suggest that LPA may exert some of its effects by regulation of SRE controlled genes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-1458476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-1643657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-1731751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-2133110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-214164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-2414012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-2551506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-2917152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-3524858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-5865378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-7679495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127683-8383331
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
450-2
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8127683-Animals,
pubmed-meshheading:8127683-Blood,
pubmed-meshheading:8127683-Calcimycin,
pubmed-meshheading:8127683-Cell Line,
pubmed-meshheading:8127683-Gene Expression Regulation,
pubmed-meshheading:8127683-Genes, fos,
pubmed-meshheading:8127683-Lysophospholipids,
pubmed-meshheading:8127683-Promoter Regions, Genetic,
pubmed-meshheading:8127683-RNA, Messenger,
pubmed-meshheading:8127683-Rats,
pubmed-meshheading:8127683-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8127683-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Activation of serum response element-regulated genes by lysophosphatidic acid.
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pubmed:affiliation |
Schering-Plough Research Institute, Kenilworth, NJ 07033.
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pubmed:publicationType |
Journal Article,
In Vitro
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