| pubmed-article:8127657 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C0010843 | lld:lifeskim |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C0311404 | lld:lifeskim |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C0205164 | lld:lifeskim |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C0285076 | lld:lifeskim |
| pubmed-article:8127657 | lifeskim:mentions | umls-concept:C0049222 | lld:lifeskim |
| pubmed-article:8127657 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8127657 | pubmed:dateCreated | 1994-4-14 | lld:pubmed |
| pubmed-article:8127657 | pubmed:abstractText | Two major stable oxidation products of 2'-deoxycytidine are 2'-deoxy-5-hydroxycytidine (5-OHdC) and 2'-deoxy-5-hydroxyuridine (5-OHdU). In order to study the in vitro incorporation of 5-OHdC and 5-OHdU into DNA by DNA polymerase, and to check the base pairing specificity of these modified bases, 5-OHdCTP and 5-OHdUTP were synthesized. Incorporation studies showed that 5-OHdCTP can replace dCTP, and to a much lesser extent dTTP, as a substrate for Escherichia coli DNA polymerase I Klenow fragment (exonuclease free). However, 5-OHdUTP can only be incorporated into DNA in place of dTTP. To study the specificity of nucleotide incorporation opposite 5-hydroxypyrimidines in template DNA, 18- and 45-member oligodeoxyribonucleotides, containing an internal 5-OHdC or 5-OHdU in two different sequence contexts, were used. Translesion synthesis past 5-OHdC and 5-OHdU in both oligonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template were observed. The specificity of nucleotide incorporation opposite 5-OHdC and 5-OHdU in the template was sequence context dependent. In one sequence context, dG was the predominant nucleotide incorporated opposite 5-OHdC with dA incorporation also observed; in this sequence context, dA was the principal nucleotide incorporated opposite 5-OHdU. However in a second sequence context, dC was the predominant base incorporated opposite 5-OHdC. In that same sequence context, dC was also the predominant nucleotide incorporated opposite 5-OHdU. These data suggest that the 5-hydroxypyrimidines have the potential to be premutagenic lesions leading to C-->T transitions and C-->G transversions. | lld:pubmed |
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| pubmed-article:8127657 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8127657 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8127657 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8127657 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8127657 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8127657 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:8127657 | pubmed:author | pubmed-author:WallaceS SSS | lld:pubmed |
| pubmed-article:8127657 | pubmed:author | pubmed-author:PurmalA AAA | lld:pubmed |
| pubmed-article:8127657 | pubmed:author | pubmed-author:KowY WYW | lld:pubmed |
| pubmed-article:8127657 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8127657 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:8127657 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:8127657 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8127657 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8127657 | pubmed:pagination | 72-8 | lld:pubmed |
| pubmed-article:8127657 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8127657 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8127657 | pubmed:articleTitle | Major oxidative products of cytosine, 5-hydroxycytosine and 5-hydroxyuracil, exhibit sequence context-dependent mispairing in vitro. | lld:pubmed |
| pubmed-article:8127657 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405-0084. | lld:pubmed |
| pubmed-article:8127657 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8127657 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8127657 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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