Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-4-14
pubmed:abstractText
Two major stable oxidation products of 2'-deoxycytidine are 2'-deoxy-5-hydroxycytidine (5-OHdC) and 2'-deoxy-5-hydroxyuridine (5-OHdU). In order to study the in vitro incorporation of 5-OHdC and 5-OHdU into DNA by DNA polymerase, and to check the base pairing specificity of these modified bases, 5-OHdCTP and 5-OHdUTP were synthesized. Incorporation studies showed that 5-OHdCTP can replace dCTP, and to a much lesser extent dTTP, as a substrate for Escherichia coli DNA polymerase I Klenow fragment (exonuclease free). However, 5-OHdUTP can only be incorporated into DNA in place of dTTP. To study the specificity of nucleotide incorporation opposite 5-hydroxypyrimidines in template DNA, 18- and 45-member oligodeoxyribonucleotides, containing an internal 5-OHdC or 5-OHdU in two different sequence contexts, were used. Translesion synthesis past 5-OHdC and 5-OHdU in both oligonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template were observed. The specificity of nucleotide incorporation opposite 5-OHdC and 5-OHdU in the template was sequence context dependent. In one sequence context, dG was the predominant nucleotide incorporated opposite 5-OHdC with dA incorporation also observed; in this sequence context, dA was the principal nucleotide incorporated opposite 5-OHdU. However in a second sequence context, dC was the predominant base incorporated opposite 5-OHdC. In that same sequence context, dC was also the predominant nucleotide incorporated opposite 5-OHdU. These data suggest that the 5-hydroxypyrimidines have the potential to be premutagenic lesions leading to C-->T transitions and C-->G transversions.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-1565630, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-1660424, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-1730583, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-1826106, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-1947004, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-1975603, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-1992344, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2055497, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2193855, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2206282, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2223758, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2682525, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2684796, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2824268, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-2966755, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-3034813, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-3088546, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-4795780, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-4846180, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-6093185, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-6326053, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-6987490, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-7683095, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127657-8479906
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
72-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Major oxidative products of cytosine, 5-hydroxycytosine and 5-hydroxyuracil, exhibit sequence context-dependent mispairing in vitro.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405-0084.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.