Linked Life Data

8126883

Source:http://linkedlifedata.com/resource/pubmed/id/8126883

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-4-8
pubmed:abstractText
The amplification of bacteria DNA by PCR followed by rapid identification with hybridization are described. In the case of Mycobacteria, a 206 bases in dnaJ gene was amplified by nested PCR with conserved primers. The amplified DNAs were then hybridized with species-specific oligoprobes. Theses oligoprobes are capable of identifying the amplified DNA as M. tuberculosis, M. avium, M. intracellulare, M. kansasii or others. In the case of MRSA, clinical samples were examined to amplify mecA, femA and tsst-1 genes in the same tube, using mixed primers and the resultant 680 bp, 307 bp and 121 bp were identified by hybridization. For Helicobacter pylori, a 203 base were amplified and identified with [32p]-labeled oligoprobe as described (Valentine, J.L. et al., J. Clin. Microbiol., 29:689-695, 1991.). The sensitivity was 10(2) CFU/tube on agarose gel and 10 CFU/tube by hybridization.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0047-1852
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
344-9
pubmed:dateRevised
2011-7-27
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Rapid identification of bacteria by PCR and hybridization].
pubmed:affiliation
Mitsubishi-Yuka Bio-Clinical Laboratories Inc., Division of Molecular Diagnosis of Infectious Disease.
pubmed:publicationType
Journal Article, English Abstract