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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1994-4-12
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pubmed:abstractText |
Flow immunophenotyping, DNA content analysis, and polymerase chain reaction (PCR) amplification for t(11;14) and t(14;18) were performed on 11 cases of typical mantle cell lymphoma (MCL), 5 cases of apparent MCL with proliferation centers (MCL-PC), and 5 cases of small lymphocytic lymphoma (SLL). Immunophenotyping showed IgM (P < .001), Ig light (P < .001), and CD20 (P < .001) expression to be more intense in MCL than in SLL. In MCL-PC, the mean intensity of IgM, Ig light chain, and CD20 expression was intermediate to the intensities observed in MCL and SLL. Furthermore, in contrast to SLL, all MCL and 4 of 5 MCL-PC cases exhibited stronger CD20 than CD19 expression. CD10 expression was not observed in any case and CD5 expression was present in all SLL and MCL-PC cases and in 9 of 11 MCL cases. DNA content analysis showed an S-phase fraction of less than 3% in all cases studied and, except for 1 MCL case, all lymphomas were DNA diploid. The t(11;14) breakpoint junctions involving the bcl-1 major translocation cluster were amplified by PCR in 4 of 11 (36%) MCL cases and in none of the MCL-PC or SLL cases. The t(14;18) involving the bcl-2 major breakpoint region was not identified by PCR in any case. We conclude that the level of expression of surface antigens and the rapid detection of t(11;14) by PCR are potentially useful for distinguishing MCL and SLL in the clinical setting. Further investigations as to the biologic relationship between MCL, MCL-PC, and SLL, and the utility of t(11;14) PCR in these lymphomas are warranted.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1626-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8123854-Aged,
pubmed-meshheading:8123854-Aged, 80 and over,
pubmed-meshheading:8123854-Antigens, Surface,
pubmed-meshheading:8123854-Base Sequence,
pubmed-meshheading:8123854-Chromosomes, Human, Pair 11,
pubmed-meshheading:8123854-Chromosomes, Human, Pair 14,
pubmed-meshheading:8123854-DNA, Neoplasm,
pubmed-meshheading:8123854-Female,
pubmed-meshheading:8123854-Flow Cytometry,
pubmed-meshheading:8123854-Humans,
pubmed-meshheading:8123854-Lymphoma, Follicular,
pubmed-meshheading:8123854-Male,
pubmed-meshheading:8123854-Middle Aged,
pubmed-meshheading:8123854-Molecular Sequence Data,
pubmed-meshheading:8123854-Polymerase Chain Reaction,
pubmed-meshheading:8123854-Translocation, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Antigen expression and polymerase chain reaction amplification of mantle cell lymphomas.
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pubmed:affiliation |
Department of Pathology, University of Utah Medical Center, Salt Lake City.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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