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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-4-4
|
| pubmed:databankReference | |
| pubmed:abstractText |
Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus, and in vitro, it can productively infect human CD4+ T cells as HIV-1. Co-infection of T cells by HIV-1 and HHV-6 can lead to both activation of the HIV-1 promoter and acceleration of the cytopathic effects. An HHV-6 (GS) cDNA clone, pCD41, encoding for a 41-kDa nuclear protein was identified and characterized previously (Chang and Balachandran, J. Virol. 65, 2884-2894 and 7085, 1991). Sequence analyses show that this protein has significant homology with the human cytomegalovirus UL44 gene coding for the ICP36 family of early-late-class phosphoprotein. Using this cDNA as the probe, a 3.8-kb EcoRI genomic fragment encoding the HHV-6(GS)P41 was cloned and designated as pGD41. When cotransfected with the HIV LTR CAT into CV-1 cells, both the pCD41 and pGD41 clones trans-activated the HIV LTR. Sequence analyses of pCD41 indicate that there are two potential open reading frames (ORFs), A and B, which are homologous to the ORFs found in the genomic clone pGD41. Deletion constructs of the pCD41 clone demonstrated that ORF-A was critical for the HIV LTR activation. Deletion analyses of the pCD41 ORF-A and the use of promoter constructs further mapped an internal functional promoter within the pCD41 sequence that can direct the synthesis of the trans-activating protein. By using HIV LTR deletion mutants, the NF-kappa B binding sites were found to be critical for response to the pCD41 trans-activation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/p41 protein, Human herpesvirus 6
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0042-6822
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
199
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
311-22
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8122364-Amino Acid Sequence,
pubmed-meshheading:8122364-Animals,
pubmed-meshheading:8122364-Base Sequence,
pubmed-meshheading:8122364-Cell Line,
pubmed-meshheading:8122364-Cercopithecus aethiops,
pubmed-meshheading:8122364-Cloning, Molecular,
pubmed-meshheading:8122364-DNA, Complementary,
pubmed-meshheading:8122364-DNA-Binding Proteins,
pubmed-meshheading:8122364-Gene Expression Regulation, Viral,
pubmed-meshheading:8122364-HIV Long Terminal Repeat,
pubmed-meshheading:8122364-HIV-1,
pubmed-meshheading:8122364-Herpesvirus 6, Human,
pubmed-meshheading:8122364-Humans,
pubmed-meshheading:8122364-Molecular Sequence Data,
pubmed-meshheading:8122364-NF-kappa B,
pubmed-meshheading:8122364-Open Reading Frames,
pubmed-meshheading:8122364-Phosphoproteins,
pubmed-meshheading:8122364-Promoter Regions, Genetic,
pubmed-meshheading:8122364-T-Lymphocytes,
pubmed-meshheading:8122364-Transcriptional Activation,
pubmed-meshheading:8122364-Viral Proteins
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
trans-activation of the HIV promoter by a cDNA and its genomic clones of human herpesvirus-6.
|
| pubmed:affiliation |
Department of Neurology and Microbiology, University of Miami School of Medicine, Florida 33101.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|