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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5151
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pubmed:dateCreated |
1994-4-4
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pubmed:abstractText |
A temperature-sensitive (ts) mutant retains the function of a gene at a low (permissive) temperature but not at a high (nonpermissive) temperature. Arg-DHFR, a dihydrofolate reductase bearing an amino-terminal (N-terminal) arginine, is long-lived in the yeast Saccharomyces cerevisiae, even though arginine is a destabilizing residue in the N-end rule of protein degradation. A ts derivative of Arg-DHFR was identified that is long-lived at 23 degrees C but rapidly degraded by the N-end rule pathway at 37 degrees C. Fusions of ts Arg-DHFR to either Ura3 or Cdc28 of S. cerevisiae confer ts phenotypes specific for these gene products. Thus, Arg-DHFRts is a heat-inducible degradation signal that can be used to produce ts mutants without a search for ts mutations.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1273-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8122109-CDC28 Protein Kinase, S cerevisiae,
pubmed-meshheading:8122109-Fungal Proteins,
pubmed-meshheading:8122109-Half-Life,
pubmed-meshheading:8122109-Hot Temperature,
pubmed-meshheading:8122109-Mutagenesis,
pubmed-meshheading:8122109-Phenotype,
pubmed-meshheading:8122109-Recombinant Fusion Proteins,
pubmed-meshheading:8122109-Saccharomyces cerevisiae,
pubmed-meshheading:8122109-Temperature,
pubmed-meshheading:8122109-Tetrahydrofolate Dehydrogenase,
pubmed-meshheading:8122109-Ubiquitins
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pubmed:year |
1994
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pubmed:articleTitle |
Heat-inducible degron: a method for constructing temperature-sensitive mutants.
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pubmed:affiliation |
Division of Biology, California Institute of Technology, Pasadena 91125.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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