Linked Life Data

8121417

Source:http://linkedlifedata.com/resource/pubmed/id/8121417

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-4-7
pubmed:databankReference
pubmed:abstractText
Many strains of Escherichia coli express type 1 pili which undergo phase variation during growth in broth, but become uniformly nonpiliated when passaged on agar media. In a previous analysis of these agar phase locked strains, we demonstrated that fimB and fimE, which mediate the site-specific DNA rearrangement involved in phase variation, are differentially transcribed under the two growth conditions. In this study the fimB promoter region was sequenced and characterized in agar phase locked strain J96. Primer extension analysis of the fimB gene identified three putative transcription initiation sites. Transcription starting from two of the sites (P1 and P2) would produce an mRNA that approximates the transcript size of fimB previously detected by Northern blot hybridizations. Gel mobility shift studies revealed the presence of promoter binding activity in broth-grown cell extracts, but not in extracts from agar-grown cells, that reacted specifically with DNA fragments upstream of the P1 and P2 promoter regions. This putative binding protein may be involved in the regulation of fimB, perhaps by acting as a transcriptional activator.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:volume
242
pubmed:geneSymbol
fimB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
623-30
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Analysis of the fimB promoter region involved in type 1 pilus phase variation in Escherichia coli.
pubmed:affiliation
Department of Microbiology-Immunology, Northwestern University, Chicago, IL 60611.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.